Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 30 mM, 197% increase in activity | Porphyromonas gingivalis | |
cysteine | 3 mM, 166% increase in activity | Porphyromonas gingivalis | |
dithiothreitol | 30 mM, 315% increase in activity | Porphyromonas gingivalis | |
EDTA | 2 mM, 79% increase in activity | Porphyromonas gingivalis | |
EGTA | 2 mM, 79% increase in activity | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | 2 mM, 65% inhibition | Porphyromonas gingivalis | |
p-hydroxymercuribenzoate | 0.2 mM, 68% inhibition | Porphyromonas gingivalis | |
tosyl-L-lysine chloromethyl ketone | 0.05 mM, 81% inhibition | Porphyromonas gingivalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell envelope | - |
Porphyromonas gingivalis | 30313 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Porphyromonas gingivalis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O | 7% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | Porphyromonas gingivalis | benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O | - |
? | |
D-Val-Phe-Lys-p-nitroanilide + H2O | 70% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | Porphyromonas gingivalis | D-Val-Phe-Lys + p-nitroaniline | - |
? | |
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis | N-p-tosyl-Gly-Pro-Lys + p-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Porphyromonas gingivalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
68% loss of activity after 10 min, 97% loss of activity after 30 min | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Porphyromonas gingivalis |