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Literature summary for 3.4.22.46 extracted from

  • Steinberger, J.; Kontaxis, G.; Rancan, C.; Skern, T.
    Comparison of self-processing of foot-and-mouth disease virus leader proteinase and porcine reproductive and respiratory syndrome virus leader proteinase nsp1alpha (2013), Virology, 443, 271-277.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Foot-and-mouth disease virus
expressed in Escherichia coli BL21(DE3)pLysS cells porcine reproductive and respiratory syndrome virus

Protein Variants

Protein Variants Comment Organism
C51A inactive Foot-and-mouth disease virus
C51A inactive porcine reproductive and respiratory syndrome virus
L200F the mutant is impaired in activity but still allows self-processing porcine reproductive and respiratory syndrome virus
L200F the mutant is impaired in but still allows self-processing Foot-and-mouth disease virus

Inhibitors

Inhibitors Comment Organism Structure
additional information self-processing inactivates leader proteinase nsp1alpha porcine reproductive and respiratory syndrome virus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19100
-
2 * 19100, calculated from amino acid sequence Foot-and-mouth disease virus
19100
-
2 * 19100, calculated from amino acid sequence porcine reproductive and respiratory syndrome virus
28800
-
2 * 28800, SDS-PAGE Foot-and-mouth disease virus
28800
-
2 * 28800, SDS-PAGE porcine reproductive and respiratory syndrome virus
41400
-
gel filtration Foot-and-mouth disease virus
41400
-
gel filtration porcine reproductive and respiratory syndrome virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eukaryotic initiation factor eIF4G + H2O Foot-and-mouth disease virus
-
?
-
?
eukaryotic initiation factor eIF4G + H2O porcine reproductive and respiratory syndrome virus
-
?
-
?
additional information Foot-and-mouth disease virus the enzyme cleaves itself off the nascent viral polyprotein ?
-
?

Organism

Organism UniProt Comment Textmining
Foot-and-mouth disease virus
-
-
-
porcine reproductive and respiratory syndrome virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitatio, Mono Q column chromatography, and Superdex 75 gel filtration porcine reproductive and respiratory syndrome virus
ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 75 gel filtration Foot-and-mouth disease virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eukaryotic initiation factor eIF4G + H2O
-
Foot-and-mouth disease virus ?
-
?
eukaryotic initiation factor eIF4G + H2O
-
porcine reproductive and respiratory syndrome virus ?
-
?
additional information the enzyme cleaves itself off the nascent viral polyprotein Foot-and-mouth disease virus ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 19100, calculated from amino acid sequence Foot-and-mouth disease virus
homodimer 2 * 19100, calculated from amino acid sequence porcine reproductive and respiratory syndrome virus
homodimer 2 * 28800, SDS-PAGE Foot-and-mouth disease virus
homodimer 2 * 28800, SDS-PAGE porcine reproductive and respiratory syndrome virus

Synonyms

Synonyms Comment Organism
Lbpro
-
Foot-and-mouth disease virus
Leader proteinase
-
Foot-and-mouth disease virus
Leader proteinase
-
porcine reproductive and respiratory syndrome virus
nsp1alpha
-
porcine reproductive and respiratory syndrome virus