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Literature summary for 3.4.22.46 extracted from

  • Xu, Y.; Li, X.; Li, R.; Li, S.; Ni, H.; Wang, H.; Xu, H.; Zhou, W.; Saris, P.E.; Yang, W.; Qiao, M.; Rao, Z.
    Structure of the nisin leader peptidase NisP revealing a C-terminal autocleavage activity (2014), Acta Crystallogr. Sect. D, 70, 1499-1505.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Lactococcus lactis strain LAC71 and in Escherichia coli BL21 (DE3) cells Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 24-32% (v/v) 1,4-dioxane Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
H306A inactive Lactococcus lactis
R647P/S648P the mutant has full catalytic activity for nisin leader-peptide cleavage, although the C-terminal region of the enzyme is no longer cleaved Lactococcus lactis
S512A inactive Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72900
-
x * 72900, enzyme with the C-terminus cleaved off, SDS-PAGE Lactococcus lactis
77500
-
x * 77500, enzyme with the C-terminus intact, SDS-PAGE Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lactococcus lactis the enzyme possesses an auto-cleavage site between residues Arg647 and Ser648 ?
-
?
nisin + H2O Lactococcus lactis the final step in nisin maturation is the proteolytic cleavage of the first 23 amino-terminal residues by NisP ?
-
?

Organism

Organism UniProt Comment Textmining
Lactococcus lactis D9IXC0
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Edans-EGAVSVRSQEIK-Dabcyl + H2O
-
Lactococcus lactis ?
-
?
additional information the enzyme possesses an auto-cleavage site between residues Arg647 and Ser648 Lactococcus lactis ?
-
?
nisin + H2O the final step in nisin maturation is the proteolytic cleavage of the first 23 amino-terminal residues by NisP Lactococcus lactis ?
-
?

Subunits

Subunits Comment Organism
? x * 72900, enzyme with the C-terminus cleaved off, SDS-PAGE Lactococcus lactis
? x * 77500, enzyme with the C-terminus intact, SDS-PAGE Lactococcus lactis

Synonyms

Synonyms Comment Organism
nisin leader peptidase
-
Lactococcus lactis
NisP
-
Lactococcus lactis

General Information

General Information Comment Organism
metabolism functions in the last step of nisin maturation as the leader-peptide peptidase Lactococcus lactis