Cloned (Comment) | Organism |
---|---|
expressed in Lactococcus lactis strain LAC71 and in Escherichia coli BL21 (DE3) cells | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 24-32% (v/v) 1,4-dioxane | Lactococcus lactis |
Protein Variants | Comment | Organism |
---|---|---|
H306A | inactive | Lactococcus lactis |
R647P/S648P | the mutant has full catalytic activity for nisin leader-peptide cleavage, although the C-terminal region of the enzyme is no longer cleaved | Lactococcus lactis |
S512A | inactive | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72900 | - |
x * 72900, enzyme with the C-terminus cleaved off, SDS-PAGE | Lactococcus lactis |
77500 | - |
x * 77500, enzyme with the C-terminus intact, SDS-PAGE | Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Lactococcus lactis | the enzyme possesses an auto-cleavage site between residues Arg647 and Ser648 | ? | - |
? | |
nisin + H2O | Lactococcus lactis | the final step in nisin maturation is the proteolytic cleavage of the first 23 amino-terminal residues by NisP | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | D9IXC0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Edans-EGAVSVRSQEIK-Dabcyl + H2O | - |
Lactococcus lactis | ? | - |
? | |
additional information | the enzyme possesses an auto-cleavage site between residues Arg647 and Ser648 | Lactococcus lactis | ? | - |
? | |
nisin + H2O | the final step in nisin maturation is the proteolytic cleavage of the first 23 amino-terminal residues by NisP | Lactococcus lactis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72900, enzyme with the C-terminus cleaved off, SDS-PAGE | Lactococcus lactis |
? | x * 77500, enzyme with the C-terminus intact, SDS-PAGE | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
nisin leader peptidase | - |
Lactococcus lactis |
NisP | - |
Lactococcus lactis |
General Information | Comment | Organism |
---|---|---|
metabolism | functions in the last step of nisin maturation as the leader-peptide peptidase | Lactococcus lactis |