Literature summary for 3.4.22.46 extracted from

Piccone, M.E.; Diaz-San Segundo, F.; Kramer, E.; Rodriguez, L.L.; de los Santos, T.
Introduction of tag epitopes in the inter-AUG region of foot and mouth disease virus: effect on the L protein (2011), Virus Res., 155, 91-97.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of FLAG- and HA-tagged FMDV A24-L1123 mutant enzyme in LF-BK cells. T7 RNA transcripts of SpeI-digested pA24-WT, pA24-L1123 and pA24-L tag mutants, as well as transcript of control plasmid pL, are translated in vitro in rabbit reticulocyte lysates. All synthesized L mutant proteins are able to cleave themselves from the precursor L-Vp4-Vp2', the Lbeta band is absent in all L mutant translation reactions. The tn insertion in these mutant L plasmids forces translation initiation in vitro from other than the second AUG codon	Foot-and-mouth disease virus

Cloned (Comment)

Organism

expression of FLAG- and HA-tagged FMDV A24-L1123 mutant enzyme in LF-BK cells. T7 RNA transcripts of SpeI-digested pA24-WT, pA24-L1123 and pA24-L tag mutants, as well as transcript of control plasmid pL, are translated in vitro in rabbit reticulocyte lysates. All synthesized L mutant proteins are able to cleave themselves from the precursor L-Vp4-Vp2', the Lbeta band is absent in all L mutant translation reactions. The tn insertion in these mutant L plasmids forces translation initiation in vitro from other than the second AUG codon

Foot-and-mouth disease virus

Protein Variants

Protein Variants	Comment	Organism
additional information	introduction of FLAG- and HA-tag on the small tetracysteine tc motif CCGPCC of cDNA-derived mutant FMDV A24-L1123, the mutant shows a small plaque phenotype similar to the parental A24-L1123 mutant. Expression of the Flag- or tc-tagged Lab protein is abolished or greatly diminished in these viruses. The A24-L1123/Flag virus acquires an extra base in the inter-AUG region that results in new AUG codons in-frame with the second AUG, and produced a larger Lb protein. This N-terminal extension of the Lb protein in mutant A24-L1123/Flag does not affect virus viability or L functions in cell culture	Foot-and-mouth disease virus

Organism

Organism	UniProt	Comment	Textmining
Foot-and-mouth disease virus	-	FMDV, two forms of the leader proteinase, Lab, starting at the first AUG, and Lb, starting at second AUG	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	the L signal is mainly localized to the nuclei of infected cells, while VP1 is detected in the cytoplasm, thereafter the L signal is detected throughout the whole cell. The pattern of L sub-cellular localization of the L mutant viruses is similar to the WT virus, except for a delay in the cytoplasmic L protein signal	Foot-and-mouth disease virus	-

Synonyms

Synonyms	Comment	Organism
L protein	-	Foot-and-mouth disease virus
L proteinase	-	Foot-and-mouth disease virus
Lab protein	-	Foot-and-mouth disease virus
Leader proteinase	-	Foot-and-mouth disease virus