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Literature summary for 3.4.22.45 extracted from
- Helper component proteinase of the genus Potyvirus is an interaction partner of translation initiation factors eIF(iso)4E and eIF4E and contains a 4E binding motif (2011), J. Virol., 85, 6784-6794.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of HCPro with YN fused to the HCpro N terminus in Nicotiana benthamiana, and translation initiation factors eIF(iso)4E (Tiso4Eb and Piso4Eb) and eIF4E (T4Ea and P4Eb) of tobacco (T) and potato (P) with YC fused to the N- or C-terminus. Leaves are infiltrated with pairs of Agrobacterium strains expressing tester proteins tagged with the opposite halves of YFP | potato virus A |
| expression of HCPro with YN fused to the HCpro N terminus in Nicotiana benthamiana, and translation initiation factors eIF(iso)4E (Tiso4Eb and Piso4Eb) and eIF4E (T4Ea and P4Eb) of tobacco (T) and potato (P) with YC fused to the N- or C-terminus. Leaves are infiltrated with pairs of Agrobacterium strains expressing tester proteins tagged with the opposite halves of YFP | tobacco etch virus |
| expression of HCPro with YN fused to the HCpro N terminus in Nicotiana benthamiana, and translation initiation factors eIF(iso)4E (Tiso4Eb and Piso4Eb) and eIF4E (T4Ea and P4Eb) of tobacco (T) and potato (P) with YC fused to the N- or C-terminus. Leaves are infiltrated with pairs of Agrobacterium strains expressing tester proteins tagged with the opposite halves of YFP. Interactions are negative in the YTHS, an artificial system in which protein interactions are tested in the nucleus | potato virus Y |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | mutations in the 4E binding site of HCpro reducing virulence of PVA | potato virus A |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | potato virus A | the helper component proteinase of potato virus A interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | ? | - |
? |  |
| additional information | potato virus Y | the helper component proteinase of potato virus Y interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | ? | - |
? | |
| additional information | tobacco etch virus | the helper component proteinase of tobacco etch virus interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | ? | - |
? | |
| additional information | tobacco etch virus TEV-HCH10 | the helper component proteinase of tobacco etch virus interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | ? | - |
? | |
| additional information | potato virus A PVA-B11 | the helper component proteinase of potato virus A interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | ? | - |
? | |
| additional information | potato virus Y PVY-Nevski | the helper component proteinase of potato virus Y interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| potato virus A | Q9DIC2 | polyprotein; PVA | - |
| potato virus A PVA-B11 | Q9DIC2 | polyprotein; PVA | - |
| potato virus Y | G2ZHC9 | polyprotein; PVY | - |
| potato virus Y PVY-Nevski | G2ZHC9 | polyprotein; PVY | - |
| tobacco etch virus | Q000U0 | polyprotein; TEV | - |
| tobacco etch virus TEV-HCH10 | Q000U0 | polyprotein; TEV | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | virus on leaves of Nicotiana benthamiana | potato virus A | - |
| additional information | virus on leaves of Nicotiana benthamiana | potato virus Y | - |
| additional information | virus on leaves of Nicotiana benthamiana | tobacco etch virus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the helper component proteinase of potato virus A interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | potato virus A | ? | - |
? | |
| additional information | the helper component proteinase of potato virus Y interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | potato virus Y | ? | - |
? | |
| additional information | the helper component proteinase of tobacco etch virus interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | tobacco etch virus | ? | - |
? | |
| additional information | the helper component proteinase of tobacco etch virus interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | tobacco etch virus TEV-HCH10 | ? | - |
? | |
| additional information | the helper component proteinase of potato virus A interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | potato virus A PVA-B11 | ? | - |
? | |
| additional information | the helper component proteinase of potato virus Y interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger, analysis by yeast two-hybrid system assay | potato virus Y PVY-Nevski | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HcPro | - |
potato virus A |
| HcPro | - |
potato virus Y |
| HcPro | - |
tobacco etch virus |
| helper component proteinase | - |
potato virus A |
| helper component proteinase | - |
potato virus Y |
| helper component proteinase | - |
tobacco etch virus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | point mutations introduced to a 4E binding motif identified in the C-terminal region of HCpro debilitate interactions of HCpro with translation initiation factors and are detrimental to the virulence of PVA in plants | potato virus A |
| physiological function | the helper component proteinase of potato virus A interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger. Roles for HCpro and/or translation factors in the potyvirus infection cycle | potato virus A |
| physiological function | the helper component proteinase of potato virus Y interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger. Roles for HCpro and/or translation factors in the potyvirus infection cycle | potato virus Y |
| physiological function | the helper component proteinase of tobacco etch virus interacts translation initiation factors with both eIF4E and eIF(iso)4E from Nicotiana tabacum and to a lesser extent from Solanum tuberosum, with interactions with eIF(iso)4E being stronger. Roles for HCpro and/or translation factors in the potyvirus infection cycle | tobacco etch virus |
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