Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | zucchini yellow mosaic virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of different N- and C-terminal truncated DELTA HC-Pro constructs, i.e. comprising residues 93-456, 114-456, and 139-456, or 1-322, 1-350, and 1-372, construction of recombinant mutant MBP:HA-HC-ProFRNK, MBP:HAHC-ProFINK, and truncated MBP:HA-HC-ProFRNK. HC-ProFRNK/FINK clearly inhibits Arabidopsis thaliana HEN1 activity in vitro. HC-ProFRNK/FINK, but not the truncated proteins HC-Pro 93-456, and HC-Pro 1-372 displaying decreased in vitro affinity for AtHEN1 binding, inhibits the methyltransferase activity of AtHEN1 in vitro | zucchini yellow mosaic virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
99000 | - |
x * 99000, recombinant MBP-tagged wild-type enzyme, SDS-PAGE | zucchini yellow mosaic virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | zucchini yellow mosaic virus | the helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Arabidopsis thaliana Hua Enhancer 1 methyltransferase, HEN1, activity in vitro | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
zucchini yellow mosaic virus | - |
ZYMV | - |
Purification (Comment) | Organism |
---|---|
recombinant MBP-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity purification using amylose magnetic beads | zucchini yellow mosaic virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Arabidopsis thaliana Hua Enhancer 1 methyltransferase, HEN1, activity in vitro | zucchini yellow mosaic virus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 99000, recombinant MBP-tagged wild-type enzyme, SDS-PAGE | zucchini yellow mosaic virus |
Synonyms | Comment | Organism |
---|---|---|
HC-Pro | - |
zucchini yellow mosaic virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | zucchini yellow mosaic virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | zucchini yellow mosaic virus |
General Information | Comment | Organism |
---|---|---|
physiological function | with respect to its silencing suppressor function, small RNA binding appears to be the major activity of HC-Pro. HC-Pro also exhibits other suppressor activities. HC-Pro inhibits the Arabidopsis thaliana Hua Enhancer 1, HEN1, activity, for suppression of plant RNA silencing as a mechanism of the pathogen for survival in the host cell, overview. The RNA methyltransferase HEN1 is responsible for the 3'-terminal 2'-O-methylation of sRNAs | zucchini yellow mosaic virus |