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Literature summary for 3.4.22.45 extracted from

  • Shiboleth, Y.M.; Haronsky, E.; Leibman, D.; Arazi, T.; Wassenegger, M.; Whitham, S.A.; Gaba, V.; Gal-On, A.
    The conserved FRNK box in HC-Pro, a plant viral suppressor of gene silencing, is required for small RNA binding and mediates symptom development (2007), J. Virol., 81, 13135-13148.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.45

Cloned(Commentary)

Cloned (Comment) Organism
binary constructs for overexpression of helper component proteinase HC-Pro in Tobacco etch virus generated, overexpression in Nicotina benthamiana via infiltration with Agrobacterium tumefaciens shown, binary vector pBin19-GFP used, pHannibal vector used for GFP-silencing inverted-repeat constructs zucchini yellow mosaic virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information zucchini yellow mosaic virus conserved FRNK Box in the helper component proteinase HC-Pro of zucchini yellow mosaic virus identified as a binding region for small RNAs and as a region associated with virus symptoms ?
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 ?
additional information zucchini yellow mosaic virus ZYMV conserved FRNK Box in the helper component proteinase HC-Pro of zucchini yellow mosaic virus identified as a binding region for small RNAs and as a region associated with virus symptoms ?
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 ?

Organism

Organism UniProt Comment Textmining
zucchini yellow mosaic virus P18479 engineered to encode six histidines within the gene of the HC–Pro
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zucchini yellow mosaic virus ZYMV P18479 engineered to encode six histidines within the gene of the HC–Pro
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Purification (Commentary)

Purification (Comment) Organism
mutant and wild-type of HC-Pro proteins, SDS-PAGE zucchini yellow mosaic virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 mutant analysis of the conserved FRNK domain in the helper component proteinase HC-Pro, mutation in the FRNK motif of HC-Pro protein attenuates symptoms after virus infection without reducing initial HC-Pro protein levels, FRNK domain identified as a probable point of contact with siRNA and miRNA duplexes, analyzed by small RNA-specific microarray, mobility shift assays and GFP suppression assay, sequence similarity of FRNK motif in potyviruses compared zucchini yellow mosaic virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information conserved FRNK Box in the helper component proteinase HC-Pro of zucchini yellow mosaic virus identified as a binding region for small RNAs and as a region associated with virus symptoms zucchini yellow mosaic virus ?
-
 ?
additional information conserved FRNK Box in the helper component proteinase HC-Pro of zucchini yellow mosaic virus identified as a binding region for small RNAs and as a region associated with virus symptoms zucchini yellow mosaic virus ZYMV ?
-
 ?

Synonyms

Synonyms Comment Organism
HC-Pro protein
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 zucchini yellow mosaic virus