Literature summary for 3.4.22.45 extracted from

Varrelmann, M.; Maiss, E.; Pilot, R.; Palkovics, L.
Use of pentapeptide-insertion scanning mutagenesis for functional mapping of the plum pox virus helper component proteinase suppressor of gene silencing (2007), J. Gen. Virol., 88, 1005-1015.

Search for more literature for 3.4.22.45

Application

Application	Comment	Organism
agriculture	pentapeptide-insertion scanning mutagenesis shown to be a tool for functional characterization of plant virus proteins	Plum pox virus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli, transformation into Agrobacterium tumefaciens C58C1 by using the binary plant expression vector pBIN61Sa	Plum pox virus

Protein Variants

Protein Variants	Comment	Organism
additional information	random-insertion scanning mutagenesis, generation of an pentapeptide-insertion scanning mutagenesis library of helper component protease HC-Pro, performed by using the mutation generation system F701 MGS, pentapeptide scanning mutants in pBIN61S-HC-Pro mapped by restriction digestion, insertion position determined by sequencing	Plum pox virus

Organism

Organism	UniProt	Comment	Textmining
Plum pox virus	P17766	genome polyprotein; PPV	-
Plum pox virus PPV	P17766	genome polyprotein; PPV	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HC-Pro protein, SDS-PAGE	Plum pox virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	characterization of functional domains of helper component protease HC-Pro involved in RNA-silencing suppression activity determined, systematic analysis of silencing suppression function of helper component protease HC-Pro using pentapeptide-insertion scanning mutagenesis presented, regions with different classes of silencing-suppression activity defined, functional map with information on structural domains, biological functions and mutations affecting silencing-suppressor function or viral synergism indicated	Plum pox virus

Synonyms

Synonyms	Comment	Organism
HC-Pro protein	-	Plum pox virus

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Release 2023.1 (January 2023)