Literature summary for 3.4.22.45 extracted from

Stenger, D.C.; Young, B.A.; French, R.
Random mutagenesis of wheat streak mosaic virus HC-Pro: non-infectious interfering mutations in a gene dispensable for systemic infection of plants (2006), J. Gen. Virol., 87, 2741-2747.

Search for more literature for 3.4.22.45

Application

Application	Comment	Organism
agriculture	functional characterization of plant virus proteins	Wheat streak mosaic virus

Cloned(Commentary)

Cloned (Comment)	Organism
fragment of pS81-SA containing the HC-Pro protein coding region used for generation of substitution mutations, ligation into pGEM5zf+ and transformation into Escherichia coli DH5a, 250 PCR-generated clones screened for mutations by single-strand conformation polymorphism SSCP analysis	Wheat streak mosaic virus

Protein Variants

Protein Variants	Comment	Organism
additional information	mutant generation within coding region of helper component protease HC-Pro protein, nine synonymous substitutions and 15 of 25 non-synonymous substitutions shown to be without phenotypic effect, four non-synonymous substitutions, including one reverting to wild type, shown to disply attenuated systemic infection, six non-synonymous substitutions and one nonsense substitution shown to display abolished systemic infectivity	Wheat streak mosaic virus

Organism

Organism	UniProt	Comment	Textmining
Wheat streak mosaic virus	-	WSMV-Sidney 81, infectious clone pS81-SA analyzed	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant HC-Pro protein, SDS-PAGE	Wheat streak mosaic virus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	infectivity assays described, GUS assay performed to assess ability of mutants of HC-Pro protein establishing primary infection foci on inoculated leaves, mutants generated of helper component protease HC-Pro protein summarized, proteinase assays performed to determine whether substitution mutations of HC-Pro protein that altered pathogenicity phenotype also affect auto-proteolysis, in all attenuated mutants retained auto-proteolytic activity of HC-Pro protein observed, activity reduced or absent among mutants unable to establish infection foci	Wheat streak mosaic virus

Synonyms

Synonyms	Comment	Organism
HC-Pro protein	-	Wheat streak mosaic virus

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Release 2023.1 (January 2023)