Literature summary for 3.4.22.45 extracted from

Young, B.A.; Hein, G.L.; French, R.; Stenger, D.C.
Substitution of conserved cysteine residues in wheat streak mosaic virus HC-Pro abolishes virus transmission by the wheat curl mite (2007), Arch. Virol., 152, 2107-2111.

Search for more literature for 3.4.22.45

Cloned(Commentary)

Cloned (Comment)	Organism
subcloned into pALTER vector for generation of substitution mutations by site-directed mutagenesis	Wheat streak mosaic virus

Protein Variants

Protein Variants	Comment	Organism
C16A	substitution mutants introduced into amino-proximal region of helper component protease HC-Pro, vector transmission abolished	Wheat streak mosaic virus
C16AC20A	double mutant generated, vector transmission abolished	Wheat streak mosaic virus
C20A	no effects on vector transmission	Wheat streak mosaic virus
C20R	no effects on vector transmission, randomized substitution	Wheat streak mosaic virus
C46A	vector transmission abolished	Wheat streak mosaic virus
C49A	vector transmission abolished	Wheat streak mosaic virus
C49AC46A	double mutant generated, vector transmission abolished	Wheat streak mosaic virus
K7N	no effects on vector transmission, randomized substitution	Wheat streak mosaic virus
N19I	no effects on vector transmission, randomized substitution	Wheat streak mosaic virus
R45K	no effects on vector transmission, randomized substitution	Wheat streak mosaic virus

Organism

Organism	UniProt	Comment	Textmining
Wheat streak mosaic virus	-	WSMV-Sidney 81, infectious clone pS81-SA analyzed	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	amino acid substitutions in the helper component protease HC-Pro evaluated for effects on virus transmission by the wheat curl mite Aceria tosichella, vector transmission shown to be abolished after alanine substitution at cysteine residues 16, 46 and 49, measurement by determination of infectivity rate	Wheat streak mosaic virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	zinc-finger like motif His13-X2-Cys16-X29-Cys46-X2-Cys49 in the helper component protease HC-Pro found to be essential for vector transmission	Wheat streak mosaic virus	?	-	?

Synonyms

Synonyms	Comment	Organism
HC-Pro protein	-	Wheat streak mosaic virus

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Release 2023.1 (January 2023)