BRENDA - Enzyme Database
show all sequences of 3.4.22.44

Structural determinants of tobacco vein mottling virus protease substrate specificity

Sun, P.; Austin, B.P.; Toezser, J.; Waugh, D.S.; Protein Sci. 19, 2240-2251 (2010)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
tobacco vein mottling virus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
purified recombinant mutant TVMV protease K65A/K67A/C151A in complex with a canonical peptide substrate RETVRFQSD, mixing of protein solution with 10 mg/ml protein with a fivefold molar excess of the peptide substrate RETVRFQSD, crystallization from a solution consisting of 0.2 M potassium formate and 20% PEG 3350, space group P212121, X-ray diffraction structure determination and analysis at 1.7 A resolution, the 20-residue C-terminus of TVMV protease is disordered, molecular replacement, using the crystal structure of TEV protease, PDB code 1Q31
tobacco vein mottling virus
Engineering
Protein Variants
Commentary
Organism
C151A
catalytically inactive mutant TVMV protease
tobacco vein mottling virus
K65A/K67A/C151A
catalytically inactive mutant TVMV protease
tobacco vein mottling virus
additional information
a truncation mutant of TVMV protease lacking the 20 C-terminal amino acid residues, TVMV1-217 protease, shows reduced activity compared to the wild-type enzyme
tobacco vein mottling virus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.082
-
TETVRFQSGTRR
recombinant wild-type TVMV protease, pH 7.0, 30C
tobacco vein mottling virus
0.108
-
TETVRFQSGTRR
recombinant mutant TVMV1-217 protease, pH 7.0, 30C
tobacco vein mottling virus
Organism
Organism
UniProt
Commentary
Textmining
tobacco vein mottling virus
Q9J0W2
TVMV
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the S3 and S4 pockets are mainly responsible for the substrate specificity, comparison to the specificity of the tobacco etch virus, TEV, protease, structural determinants of substrate specificity, overview. The TVMV protease is less tolerant of variation at the P1' position than TEV protease, and substitutions in the P6 position are more readily tolerated by TVMV than TEV protease
718366
tobacco vein mottling virus
?
-
-
-
-
RETVRFQSD + H2O
-
718366
tobacco vein mottling virus
?
-
-
-
?
TETVRFQSGTRR + H2O
-
718366
tobacco vein mottling virus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
three-dimensional structure of TVMV protease, overview
tobacco vein mottling virus
Synonyms
Synonyms
Commentary
Organism
tobacco vein mottling virus protease
-
tobacco vein mottling virus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
tobacco vein mottling virus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.092
-
TETVRFQSGTRR
recombinant wild-type TVMV protease, pH 7.0, 30C
tobacco vein mottling virus
0.094
-
TETVRFQSGTRR
recombinant mutant TVMV1-217 protease, pH 7.0, 30C
tobacco vein mottling virus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
tobacco vein mottling virus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
tobacco vein mottling virus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant mutant TVMV protease K65A/K67A/C151A in complex with a canonical peptide substrate RETVRFQSD, mixing of protein solution with 10 mg/ml protein with a fivefold molar excess of the peptide substrate RETVRFQSD, crystallization from a solution consisting of 0.2 M potassium formate and 20% PEG 3350, space group P212121, X-ray diffraction structure determination and analysis at 1.7 A resolution, the 20-residue C-terminus of TVMV protease is disordered, molecular replacement, using the crystal structure of TEV protease, PDB code 1Q31
tobacco vein mottling virus
Engineering (protein specific)
Protein Variants
Commentary
Organism
C151A
catalytically inactive mutant TVMV protease
tobacco vein mottling virus
K65A/K67A/C151A
catalytically inactive mutant TVMV protease
tobacco vein mottling virus
additional information
a truncation mutant of TVMV protease lacking the 20 C-terminal amino acid residues, TVMV1-217 protease, shows reduced activity compared to the wild-type enzyme
tobacco vein mottling virus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.082
-
TETVRFQSGTRR
recombinant wild-type TVMV protease, pH 7.0, 30C
tobacco vein mottling virus
0.108
-
TETVRFQSGTRR
recombinant mutant TVMV1-217 protease, pH 7.0, 30C
tobacco vein mottling virus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the S3 and S4 pockets are mainly responsible for the substrate specificity, comparison to the specificity of the tobacco etch virus, TEV, protease, structural determinants of substrate specificity, overview. The TVMV protease is less tolerant of variation at the P1' position than TEV protease, and substitutions in the P6 position are more readily tolerated by TVMV than TEV protease
718366
tobacco vein mottling virus
?
-
-
-
-
RETVRFQSD + H2O
-
718366
tobacco vein mottling virus
?
-
-
-
?
TETVRFQSGTRR + H2O
-
718366
tobacco vein mottling virus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
three-dimensional structure of TVMV protease, overview
tobacco vein mottling virus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
tobacco vein mottling virus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.092
-
TETVRFQSGTRR
recombinant wild-type TVMV protease, pH 7.0, 30C
tobacco vein mottling virus
0.094
-
TETVRFQSGTRR
recombinant mutant TVMV1-217 protease, pH 7.0, 30C
tobacco vein mottling virus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
tobacco vein mottling virus
General Information
General Information
Commentary
Organism
physiological function
the TVMV genome is translated into a single large polyprotein that is subsequently processed by three virally encoded proteases. Seven of the nine cleavage events are carried out by the NIa protease
tobacco vein mottling virus
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the TVMV genome is translated into a single large polyprotein that is subsequently processed by three virally encoded proteases. Seven of the nine cleavage events are carried out by the NIa protease
tobacco vein mottling virus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.87
-
TETVRFQSGTRR
recombinant mutant TVMV1-217 protease, pH 7.0, 30C
tobacco vein mottling virus
1.12
-
TETVRFQSGTRR
recombinant wild-type TVMV protease, pH 7.0, 30C
tobacco vein mottling virus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.87
-
TETVRFQSGTRR
recombinant mutant TVMV1-217 protease, pH 7.0, 30C
tobacco vein mottling virus
1.12
-
TETVRFQSGTRR
recombinant wild-type TVMV protease, pH 7.0, 30C
tobacco vein mottling virus
Other publictions for EC 3.4.22.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
754840
Bak
A viral protease relocalizes ...
turnip mosaic virus
Nat. Commun.
8
14493
2017
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1
1
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753096
Martinez
Interaction network of tobacc ...
tobacco etch virus
BMC Genomics
17
87
2016
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7
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1
1
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732630
Casteel
The NIa-Pro protein of Turnip ...
turnip mosaic virus
Plant J.
77
653-663
2014
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1
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1
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11
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1
1
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732794
Shin
Intracellular cleavage of amyl ...
turnip mosaic virus
PLoS ONE
9
e98650
2014
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1
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3
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1
1
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718449
Mathur
Functional regulation of PVBV ...
Chilli veinal mottle virus
Virology
422
254-264
2012
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1
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5
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8
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1
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1
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2
1
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1
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1
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5
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8
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2
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1
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8
1
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1
1
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-
731098
Gao
A set of host proteins interac ...
papaya ringspot virus
Acta Virol.
56
25-30
2012
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1
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2
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5
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2
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731247
Mathur
Novel ATPase activity of the p ...
Chilli veinal mottle virus
Biochem. Biophys. Res. Commun.
427
113-118
2012
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1
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1
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3
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1
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1
1
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731765
Kim
Expression of the plant viral ...
turnip mosaic virus
Exp. Mol. Med.
44
740-748
2012
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1
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1
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4
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1
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732853
Zheng
An effective system for detect ...
Plum pox virus
Protein Cell
3
921-928
2012
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718264
Han
The nuclear inclusion a (NIa) ...
turnip mosaic virus
PLoS ONE
5
e15645
2010
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1
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1
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2
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718366
Sun
Structural determinants of tob ...
tobacco vein mottling virus
Protein Sci.
19
2240-2251
2010
-
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1
1
3
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2
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6
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683103
Farreyrol
Vanilla mosaic virus isolates ...
Vanilla mosaic virus
Arch. Virol.
151
905-919
2006
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652663
Kang
Determination of the substrate ...
turnip mosaic potyvirus, turnip mosaic potyvirus TuMV
J. Gen. Virol.
82
3115-3117
2001
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137278
Hwang
Molecular cloning, expression, ...
tobacco vein mottling virus, tobacco vein mottling virus TVMV
Mol. Cells
10
148-155
2000
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9
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6
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2
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137273
Fellers
-
The NIa-proteinase of differen ...
potato virus Y, potato virus Y PVY, tobacco etch virus, tobacco etch virus TEV, tobacco vein mottling virus, tobacco vein mottling virus TVMV
Crop Sci.
38
1309-1319
1998
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3
3
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137274
Kim
-
Potyvirus NIa protease ...
potato virus Y, potato virus Y PVY, tobacco etch virus, tobacco etch virus TEV, tobacco vein mottling virus, tobacco vein mottling virus TVMV
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
721-723
1998
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11
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137275
Takahashi
Direct formation of human inte ...
clover yellow vein virus, clover yellow vein virus ClYVV
Biosci. Biotechnol. Biochem.
62
953-958
1998
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137276
Kim
Effects of internal cleavages ...
turnip mosaic potyvirus, turnip mosaic potyvirus TuMV
Virology
226
183-190
1996
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5
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1
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2
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2
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2
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2
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4
-
1
-
1
2
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137277
Kim
Characterization of NIa protea ...
turnip mosaic potyvirus, turnip mosaic potyvirus TuMV
Virology
221
245-249
1996
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6
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2
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4
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4
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1
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6
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6
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2
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4
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1
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6
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137279
Parks
Expression and purification of ...
tobacco etch virus, tobacco etch virus TEV
Virology
210
194-201
1995
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1
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2
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2
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4
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1
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4
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2
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1
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2
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2
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1
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-
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4
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2
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137280
Wisler
Characterization of the P1 pro ...
zucchini yellow mosaic virus, zucchini yellow mosaic virus ZYMV
J. Gen. Virol.
76
37-45
1995
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1
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2
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137281
Garcia
Inhibitory effects of human cy ...
Plum pox virus, Plum pox virus PPV
Plant Mol. Biol.
22
697-701
1993
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2
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2
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2
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2
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-
-
2
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137282
Carrington
Internal cleavage and trans-pr ...
tobacco etch virus
J. Virol.
67
6995-7000
1993
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1
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1
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1
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4
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1
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1
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1
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1
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1
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137283
Vardi
Plants transformed with a cist ...
potato virus Y
Proc. Natl. Acad. Sci. USA
90
7513-7517
1993
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1
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1
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1
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137284
Rorrer
Autocatalytic activity of the ...
tobacco etch virus
J. Gen. Virol.
73
775-783
1992
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1
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3
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1
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1
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1
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137285
Garcia
Proteolytic processing of the ...
Plum pox virus
Virology
188
697-703
1992
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1
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1
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3
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1
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1
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1
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1
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137286
Parks
Substrate recognition by the N ...
tobacco etch virus, tobacco vein mottling virus
Virology
182
17-27
1991
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-
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2
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6
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2
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2
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2
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137287
Garcia
Proteolytic activity of the pl ...
Plum pox virus
FEBS Lett.
257
269-273
1989
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-
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1
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1
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3
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1
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1
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1
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1
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