BRENDA - Enzyme Database show
show all sequences of 3.4.22.44

The nuclear inclusion a (NIa) protease of turnip mosaic virus (TuMV) cleaves amyloid-beta

Han, H.E.; Sellamuthu, S.; Shin, B.H.; Lee, Y.J.; Song, S.; Seo, J.S.; Baek, I.S.; Bae, J.; Kim, H.; Yoo, Y.J.; Jung, Y.K.; Song, W.K.; Han, P.L.; Park, W.J.; PLoS ONE 5, e15645 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
cloning of NIa in Escherichia coli, overexpression of HA-tagged NIa in rat B103 neuroblastoma cells
turnip mosaic virus
Engineering
Amino acid exchange
Commentary
Organism
additional information
overexpression of HA-tagged NIa in rat B103 neuroblastoma cells and lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice or human 293T cells, the pattern of NIa expression shows a wide distribution throughout the mouse brain including the cerebral cortex, hippocampus, amygdala, and thalamus, and the Amyloid-beta deposition in the prefrontal cortex, parietal cortex, hippocampus and piriform cortex is remarkably decreased in the brain infused with Lenti-NIa in comparison to the brain infused with Lenti-GFP. Expression of wild-type and utant, with an Asp to Ala substitution in the catalytic triad,.Amyloid-beta intracellularly in B103 cells using the plasmid pGFPUb-Abeta, encoding a triple fusion protein of green fluorescent protein, ubiquitin, and Amylod-beta. The peptide bond between ubiquitin and Amyloid-beta is cleaved quickly by endogenous deubiquitinating enzymes, generating an equimolar ratio of GFP-ubiquitin and Amyloid-beta in the cytosol
turnip mosaic virus
Inhibitors
Inhibitors
Commentary
Organism
Structure
NEM
does not completely inhibit NIa activity
turnip mosaic virus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
predominantly
turnip mosaic virus
5829
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
turnip mosaic virus
-
TuMV
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amyloid-beta peptide + H2O
degradation of oligomeric as well as monomeric forms of Amyloid-beta, presence of the consensus sequence, Val12-His-His-Gln15, near the presumptive alpha-secretase cleavage site of the amyloid-beta peptide
718264
turnip mosaic virus
?
-
-
-
?
additional information
NIa possesses a relatively strict substrate specificity with a preference for Val-Xaa-His-GlnQ, with the scissile bond located after Gln
718264
turnip mosaic virus
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
turnip mosaic virus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
turnip mosaic virus
Cloned(Commentary) (protein specific)
Commentary
Organism
cloning of NIa in Escherichia coli, overexpression of HA-tagged NIa in rat B103 neuroblastoma cells
turnip mosaic virus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
overexpression of HA-tagged NIa in rat B103 neuroblastoma cells and lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice or human 293T cells, the pattern of NIa expression shows a wide distribution throughout the mouse brain including the cerebral cortex, hippocampus, amygdala, and thalamus, and the Amyloid-beta deposition in the prefrontal cortex, parietal cortex, hippocampus and piriform cortex is remarkably decreased in the brain infused with Lenti-NIa in comparison to the brain infused with Lenti-GFP. Expression of wild-type and utant, with an Asp to Ala substitution in the catalytic triad,.Amyloid-beta intracellularly in B103 cells using the plasmid pGFPUb-Abeta, encoding a triple fusion protein of green fluorescent protein, ubiquitin, and Amylod-beta. The peptide bond between ubiquitin and Amyloid-beta is cleaved quickly by endogenous deubiquitinating enzymes, generating an equimolar ratio of GFP-ubiquitin and Amyloid-beta in the cytosol
turnip mosaic virus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NEM
does not completely inhibit NIa activity
turnip mosaic virus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
predominantly
turnip mosaic virus
5829
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amyloid-beta peptide + H2O
degradation of oligomeric as well as monomeric forms of Amyloid-beta, presence of the consensus sequence, Val12-His-His-Gln15, near the presumptive alpha-secretase cleavage site of the amyloid-beta peptide
718264
turnip mosaic virus
?
-
-
-
?
additional information
NIa possesses a relatively strict substrate specificity with a preference for Val-Xaa-His-GlnQ, with the scissile bond located after Gln
718264
turnip mosaic virus
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
turnip mosaic virus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
turnip mosaic virus
General Information
General Information
Commentary
Organism
additional information
overexpression of NIa in rat B103 neuroblastoma cells results in a significant reduction in cell death caused by both intracellularly generated and exogenously added Amyloidbeta. Moreover, lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice significantly reduces the levels of Amyloid-beta and plaques in the brain
turnip mosaic virus
physiological function
the nuclear inclusion a protease of turnip mosaic virus is responsible for the processing of the viral polyprotein into functional proteins. Degradation of Amyloid-beta in the cytoplasm can be a novel strategy to control the levels of Amyloid-beta, plaque formation, and the associated cell death
turnip mosaic virus
General Information (protein specific)
General Information
Commentary
Organism
additional information
overexpression of NIa in rat B103 neuroblastoma cells results in a significant reduction in cell death caused by both intracellularly generated and exogenously added Amyloidbeta. Moreover, lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice significantly reduces the levels of Amyloid-beta and plaques in the brain
turnip mosaic virus
physiological function
the nuclear inclusion a protease of turnip mosaic virus is responsible for the processing of the viral polyprotein into functional proteins. Degradation of Amyloid-beta in the cytoplasm can be a novel strategy to control the levels of Amyloid-beta, plaque formation, and the associated cell death
turnip mosaic virus
Other publictions for EC 3.4.22.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732630
Casteel
The NIa-Pro protein of Turnip ...
turnip mosaic virus
Plant J.
77
653-663
2014
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732794
Shin
Intracellular cleavage of amyl ...
turnip mosaic virus
PLoS ONE
9
e98650
2014
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718449
Mathur
Functional regulation of PVBV ...
Chilli veinal mottle virus
Virology
422
254-264
2012
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5
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8
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731098
Gao
A set of host proteins interac ...
papaya ringspot virus
Acta Virol.
56
25-30
2012
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1
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731247
Mathur
Novel ATPase activity of the p ...
Chilli veinal mottle virus
Biochem. Biophys. Res. Commun.
427
113-118
2012
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731765
Kim
Expression of the plant viral ...
turnip mosaic virus
Exp. Mol. Med.
44
740-748
2012
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4
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732853
Zheng
An effective system for detect ...
Plum pox virus
Protein Cell
3
921-928
2012
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1
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718264
Han
The nuclear inclusion a (NIa) ...
turnip mosaic virus
PLoS ONE
5
e15645
2010
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1
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2
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718366
Sun
Structural determinants of tob ...
tobacco vein mottling virus
Protein Sci.
19
2240-2251
2010
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1
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2
683103
Farreyrol
Vanilla mosaic virus isolates ...
Vanilla mosaic virus
Arch. Virol.
151
905-919
2006
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652663
Kang
Determination of the substrate ...
turnip mosaic potyvirus, turnip mosaic potyvirus TuMV
J. Gen. Virol.
82
3115-3117
2001
-
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6
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4
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137278
Hwang
Molecular cloning, expression, ...
tobacco vein mottling virus, tobacco vein mottling virus TVMV
Mol. Cells
10
148-155
2000
-
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1
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2
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2
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9
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1
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6
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2
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137273
Fellers
-
The NIa-proteinase of differen ...
potato virus Y, potato virus Y PVY, tobacco etch virus, tobacco etch virus TEV, tobacco vein mottling virus, tobacco vein mottling virus TVMV
Crop Sci.
38
1309-1319
1998
-
3
3
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11
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137274
Kim
-
Potyvirus NIa protease ...
potato virus Y, potato virus Y PVY, tobacco etch virus, tobacco etch virus TEV, tobacco vein mottling virus, tobacco vein mottling virus TVMV
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
721-723
1998
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137275
Takahashi
Direct formation of human inte ...
clover yellow vein virus, clover yellow vein virus ClYVV
Biosci. Biotechnol. Biochem.
62
953-958
1998
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1
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137276
Kim
Effects of internal cleavages ...
turnip mosaic potyvirus, turnip mosaic potyvirus TuMV
Virology
226
183-190
1996
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137277
Kim
Characterization of NIa protea ...
turnip mosaic potyvirus, turnip mosaic potyvirus TuMV
Virology
221
245-249
1996
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137279
Parks
Expression and purification of ...
tobacco etch virus, tobacco etch virus TEV
Virology
210
194-201
1995
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137280
Wisler
Characterization of the P1 pro ...
zucchini yellow mosaic virus, zucchini yellow mosaic virus ZYMV
J. Gen. Virol.
76
37-45
1995
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1
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137281
Garcia
Inhibitory effects of human cy ...
Plum pox virus, Plum pox virus PPV
Plant Mol. Biol.
22
697-701
1993
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137282
Carrington
Internal cleavage and trans-pr ...
tobacco etch virus
J. Virol.
67
6995-7000
1993
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