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Literature summary for 3.4.22.44 extracted from
- The nuclear inclusion a (NIa) protease of turnip mosaic virus (TuMV) cleaves amyloid-beta (2010), PLoS ONE, 5, e15645.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning of NIa in Escherichia coli, overexpression of HA-tagged NIa in rat B103 neuroblastoma cells | turnip mosaic virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | overexpression of HA-tagged NIa in rat B103 neuroblastoma cells and lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice or human 293T cells, the pattern of NIa expression shows a wide distribution throughout the mouse brain including the cerebral cortex, hippocampus, amygdala, and thalamus, and the Amyloid-beta deposition in the prefrontal cortex, parietal cortex, hippocampus and piriform cortex is remarkably decreased in the brain infused with Lenti-NIa in comparison to the brain infused with Lenti-GFP. Expression of wild-type and utant, with an Asp to Ala substitution in the catalytic triad,.Amyloid-beta intracellularly in B103 cells using the plasmid pGFPUb-Abeta, encoding a triple fusion protein of green fluorescent protein, ubiquitin, and Amylod-beta. The peptide bond between ubiquitin and Amyloid-beta is cleaved quickly by endogenous deubiquitinating enzymes, generating an equimolar ratio of GFP-ubiquitin and Amyloid-beta in the cytosol | turnip mosaic virus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | does not completely inhibit NIa activity | turnip mosaic virus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | predominantly | turnip mosaic virus | 5829 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| turnip mosaic virus | - |
TuMV | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amyloid-beta peptide + H2O | degradation of oligomeric as well as monomeric forms of Amyloid-beta, presence of the consensus sequence, Val12-His-His-Gln15, near the presumptive alpha-secretase cleavage site of the amyloid-beta peptide | turnip mosaic virus | ? | - |
? | |
| additional information | NIa possesses a relatively strict substrate specificity with a preference for Val-Xaa-His-GlnQ, with the scissile bond located after Gln | turnip mosaic virus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NIa protease | - |
turnip mosaic virus |
| nuclear inclusion a protease | - |
turnip mosaic virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | turnip mosaic virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | turnip mosaic virus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | overexpression of NIa in rat B103 neuroblastoma cells results in a significant reduction in cell death caused by both intracellularly generated and exogenously added Amyloidbeta. Moreover, lentiviral-mediated expression of NIa in APPsw/PS1 transgenic mice significantly reduces the levels of Amyloid-beta and plaques in the brain | turnip mosaic virus |
| physiological function | the nuclear inclusion a protease of turnip mosaic virus is responsible for the processing of the viral polyprotein into functional proteins. Degradation of Amyloid-beta in the cytoplasm can be a novel strategy to control the levels of Amyloid-beta, plaque formation, and the associated cell death | turnip mosaic virus |
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Release 2023.1 (January 2023)
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