Cloned (Comment) | Organism |
---|---|
recombinant human cathepsin V produced using the Pichia pastoris expression system | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | Homo sapiens | capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O60911 | - |
- |
Purification (Comment) | Organism |
---|---|
gel filtration, recombinant human cathepsin V | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HUVEC cell | plasminogen processing by cathepsin V, angiogenesis inhibition activity determined in | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
plasminogen digestion by cathepsin V determined by SDS-PAGE and by subsequent plasmin activity tests, sequences EKKVYL, TEQLAP and LLPNVE obtained by N-terminal sequencing compatible with cleavage sites at plasminogen F94-E95, S358-T359 and V468-L469 peptide bonds, in vitro angiogenesis Matrigel assay described, angiogenesis inhibition activity caused by plasminogen processing by cathepsin V determined | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
plasminogen + H2O | capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested | Homo sapiens | ? | - |
? | |
plasminogen + H2O | 4.5 microM human plasminogen and 30 nM of recombinant human cathepsins V used for plasminogen digestion assay, three major products of 60, 50 and 40 kDa obtained, SDS-PAGE | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
plasminogen digestion assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
plasminogen digestion assay at | Homo sapiens |