BRENDA - Enzyme Database show
show all sequences of 3.4.22.43

Cathepsin V, but not cathepsins L, B and K, may release angiostatin-like fragments from plasminogen

Puzer, L.; Barros, N.M.; Paschoalin, T.; Hirata, I.Y.; Tanaka, A.S.; Oliveira, M.C.; Broemme, D.; Carmona, A.K.; Biol. Chem. 389, 195-200 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
recombinant human cathepsin V produced using the Pichia pastoris expression system
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
plasminogen + H2O
Homo sapiens
capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
O60911
-
-
Purification (Commentary)
Commentary
Organism
gel filtration, recombinant human cathepsin V
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HUVEC cell
plasminogen processing by cathepsin V, angiogenesis inhibition activity determined in
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
plasminogen digestion by cathepsin V determined by SDS-PAGE and by subsequent plasmin activity tests, sequences EKKVYL, TEQLAP and LLPNVE obtained by N-terminal sequencing compatible with cleavage sites at plasminogen F94-E95, S358-T359 and V468-L469 peptide bonds, in vitro angiogenesis Matrigel assay described, angiogenesis inhibition activity caused by plasminogen processing by cathepsin V determined
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
plasminogen + H2O
capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested
683210
Homo sapiens
?
-
-
-
?
plasminogen + H2O
4.5 microM human plasminogen and 30 nM of recombinant human cathepsins V used for plasminogen digestion assay, three major products of 60, 50 and 40 kDa obtained, SDS-PAGE
683210
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
plasminogen digestion assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
plasminogen digestion assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
recombinant human cathepsin V produced using the Pichia pastoris expression system
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
plasminogen + H2O
Homo sapiens
capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested
?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
gel filtration, recombinant human cathepsin V
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HUVEC cell
plasminogen processing by cathepsin V, angiogenesis inhibition activity determined in
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
plasminogen digestion by cathepsin V determined by SDS-PAGE and by subsequent plasmin activity tests, sequences EKKVYL, TEQLAP and LLPNVE obtained by N-terminal sequencing compatible with cleavage sites at plasminogen F94-E95, S358-T359 and V468-L469 peptide bonds, in vitro angiogenesis Matrigel assay described, angiogenesis inhibition activity caused by plasminogen processing by cathepsin V determined
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
plasminogen + H2O
capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested
683210
Homo sapiens
?
-
-
-
?
plasminogen + H2O
4.5 microM human plasminogen and 30 nM of recombinant human cathepsins V used for plasminogen digestion assay, three major products of 60, 50 and 40 kDa obtained, SDS-PAGE
683210
Homo sapiens
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
plasminogen digestion assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
plasminogen digestion assay at
Homo sapiens
Other publictions for EC 3.4.22.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732591
Wong
CTSL2 is a pro-apoptotic targe ...
Homo sapiens
Oncogene
33
1249-1257
2014
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5
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1
2
2
1
-
-
731213
McCarthy
-
Cathepsin L proteases of the p ...
Lepeophtheirus salmonis
Aquaculture
356-357
264-271
2012
-
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1
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1
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1
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731573
Skrzypczak
Expression of cysteine proteas ...
Homo sapiens
Cancer Invest.
30
398-403
2012
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-
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2
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2
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2
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1
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1
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-
732049
Funkelstein
Human cathepsin V protease par ...
Homo sapiens
J. Biol. Chem.
287
15232-15241
2012
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5
1
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1
1
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732502
Keegan
Tumor necrosis factor alpha st ...
Homo sapiens
Mol. Cell. Biochem.
367
65-72
2012
-
-
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1
1
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2
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1
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3
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1
1
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1
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1
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732867
Novinec
A simple and efficient protoco ...
Homo sapiens
Protein Expr. Purif.
82
1-5
2012
1
-
1
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1
2
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1
1
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2
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1
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1
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1
1
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1
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4
1
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2
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2
2
717159
Wilder
Manipulating substrate and pH ...
Homo sapiens
Arch. Biochem. Biophys.
516
52-57
2011
-
-
1
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1
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2
1
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2
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1
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1
1
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1
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1
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1
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1
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1
1
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1
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717374
Severino
Acridone alkaloids as potent i ...
Homo sapiens
Bioorg. Med. Chem.
19
1477-1481
2011
-
-
1
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-
-
11
-
1
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2
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1
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9
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11
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1
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11
11
9
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1
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1
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717375
Piovan
Structure-activity relationshi ...
Homo sapiens
Bioorg. Med. Chem.
19
2009-2014
2011
-
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6
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1
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1
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6
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1
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707590
Elie
Identification and pre-clinica ...
Homo sapiens
Biochimie
92
1618-1624
2010
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
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1
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1
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1
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-
707591
Sevenich
Expression of human cathepsin ...
Homo sapiens
Biochimie
92
1674-1680
2010
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
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4
-
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1
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1
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1
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4
-
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1
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1
1
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707626
Coppini
Plasminogen hydrolysis by cath ...
Homo sapiens
Biol. Chem.
391
561-570
2010
-
-
1
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-
-
-
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-
-
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1
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1
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1
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7
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1
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1
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1
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7
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6
6
709133
Renko
Versatile loops in mycocypins ...
Homo sapiens
J. Biol. Chem.
285
308-316
2010
-
-
1
1
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2
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3
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1
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2
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707906
Cheng
The cystatin M/E-controlled pa ...
Homo sapiens
Br. J. Dermatol.
161
253-264
2009
-
-
-
-
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1
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-
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3
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18
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1
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18
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1
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-
1
-
-
709405
Zeeuwen
The biology of cystatin M/E an ...
Homo sapiens
J. Invest. Dermatol.
129
1327-1338
2009
-
-
-
-
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1
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3
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3
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1
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3
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1
1
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683210
Puzer
Cathepsin V, but not cathepsin ...
Homo sapiens
Biol. Chem.
389
195-200
2008
-
-
1
-
-
-
-
-
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1
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2
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1
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1
1
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1
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1
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1
1
-
2
-
1
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-
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1
-
-
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-
-
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-
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-
698753
Mihelic
Inhibitory fragment from the p ...
Homo sapiens
J. Biol. Chem.
283
14453-14460
2008
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1
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1
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1
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683207
Burden
Inhibition of cathepsin L-like ...
Homo sapiens
Biol. Chem.
388
541-545
2007
-
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1
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1
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2
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1
1
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1
1
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1
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1
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683662
Ong
DNA accelerates the inhibition ...
Homo sapiens
J. Biol. Chem.
282
36980-36986
2007
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1
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2
1
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2
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1
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1
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1
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1
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1
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1
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1
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1
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1
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1
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669460
Cheng
Cystatin M/E is a high affinit ...
Homo sapiens
J. Biol. Chem.
281
15893-15899
2006
-
-
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-
-
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1
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2
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-
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2
-
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2
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1
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1
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2
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1
2
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-
-
2
-
-
2
-
1
-
-
-
1
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-
-
-
-
-
-
-
-
669754
Zeeuwen
Colocalization of cystatin M/E ...
Homo sapiens
J. Invest. Dermatol.
127
120-128
2006
-
-
-
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1
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1
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3
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4
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1
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1
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1
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4
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1
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-
-
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683107
Helske
Increased Expression of Elasto ...
Homo sapiens
Arterioscler. Thromb. Vasc. Biol.
26
1791-1798
2006
-
1
-
-
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-
1
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3
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2
1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
669009
Kenney
Increased levels of catalase a ...
Homo sapiens
Invest. Ophthalmol. Vis. Sci.
46
823-832
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
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-
-
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-
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-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667305
Puzer
Comparative substrate specific ...
Homo sapiens
Arch. Biochem. Biophys.
430
274-283
2004
-
-
-
-
-
-
-
8
-
-
-
-
-
2
-
-
-
-
-
-
-
-
12
-
1
-
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
12
-
1
-
-
8
1
-
-
-
-
-
-
-
-
-
668472
Hagemann
The human cysteine protease ca ...
Homo sapiens
Eur. J. Cell Biol.
83
775-780
2004
-
-
1
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668763
Broemme
-
Cathepsin V ...
Homo sapiens
Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds)
2nd Ed.
1107-1109
2004
-
1
1
-
-
-
5
-
1
-
3
-
-
1
-
2
1
-
-
5
-
-
6
2
-
-
-
-
1
1
2
-
-
-
-
-
1
1
-
-
-
-
-
5
-
-
1
-
3
-
-
-
2
1
-
5
-
-
6
2
-
-
-
-
1
1
2
-
-
-
-
-
-
-
669257
Yasuda
Cathepsin V, a novel and poten ...
Homo sapiens
J. Biol. Chem.
279
36761-36770
2004
-
-
1
-
-
-
4
2
1
1
-
1
-
3
-
1
1
-
-
5
-
-
4
-
1
-
1
2
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
4
-
2
1
1
-
1
-
-
1
1
-
5
-
-
4
-
1
-
1
2
1
-
1
-
-
-
-
-
-
-
137272
Somoza
Crystal structure of human cat ...
Homo sapiens
Biochemistry
39
12543-12551
2000
-
-
1
1
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137269
Brömme
Human cathepsin V functional e ...
Homo sapiens
Biochemistry
38
2377-2385
1999
-
-
1
-
-
-
7
-
2
-
1
-
-
4
-
1
-
-
-
4
-
-
1
-
-
-
-
-
1
1
3
-
-
-
-
-
-
1
-
-
-
-
-
7
-
-
2
-
1
-
-
-
1
-
-
4
-
-
1
-
-
-
-
-
1
1
3
-
-
-
-
-
-
-
137270
Adachi
Isolation and characterization ...
Homo sapiens
Invest. Ophthalmol. Vis. Sci.
39
1789-1796
1998
-
-
1
-
-
-
2
-
-
-
5
1
-
3
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
5
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
137271
Santamaria
Cathepsin L2, a novel human cy ...
Homo sapiens
Cancer Res.
58
1624-1630
1998
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
1
-
-
-
5
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
5
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-