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Literature summary for 3.4.22.43 extracted from

  • Puzer, L.; Barros, N.M.; Paschoalin, T.; Hirata, I.Y.; Tanaka, A.S.; Oliveira, M.C.; Broemme, D.; Carmona, A.K.
    Cathepsin V, but not cathepsins L, B and K, may release angiostatin-like fragments from plasminogen (2008), Biol. Chem., 389, 195-200.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant human cathepsin V produced using the Pichia pastoris expression system Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plasminogen + H2O Homo sapiens capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60911
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration, recombinant human cathepsin V Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HUVEC cell plasminogen processing by cathepsin V, angiogenesis inhibition activity determined in Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
plasminogen digestion by cathepsin V determined by SDS-PAGE and by subsequent plasmin activity tests, sequences EKKVYL, TEQLAP and LLPNVE obtained by N-terminal sequencing compatible with cleavage sites at plasminogen F94-E95, S358-T359 and V468-L469 peptide bonds, in vitro angiogenesis Matrigel assay described, angiogenesis inhibition activity caused by plasminogen processing by cathepsin V determined Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
plasminogen + H2O capability of recombinant human cathepsin V to hydrolyze plasminogen analyzed, physiological role for cathepsin V related to the control of neovascularization in cornea suggested Homo sapiens ?
-
?
plasminogen + H2O 4.5 microM human plasminogen and 30 nM of recombinant human cathepsins V used for plasminogen digestion assay, three major products of 60, 50 and 40 kDa obtained, SDS-PAGE Homo sapiens ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
plasminogen digestion assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
plasminogen digestion assay at Homo sapiens