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Literature summary for 3.4.22.43 extracted from

  • Burden, R.E.; Snoddy, P.; Jefferies, C.A.; Walker, B.; Scott, C.J.
    Inhibition of cathepsin L-like proteases by cathepsin V propeptide (2007), Biol. Chem., 388, 541-545.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cathepsin V propeptide CatV PP, tight-binding inhibitor Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O60911
-
-

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation recombinant human cathepsin V for inhibition studies, N-terminal propeptide domains as inhibitors of cathepsin V Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
inhibition properties of cathepsin V propeptide by fluorimetric assay determined, purification and expression of cathepsin V propeptide described, recombinant cathepsin V used for inhibition studies Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O Cbz-Phe-Arg-AMC, 25 microM used for protease inhibition assay, inhibitory conditions of cathepsin V propeptide analyzed Homo sapiens N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
protease inhibition assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0102
-
cathepsin V propeptide varying concentrations tested Homo sapiens