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Literature summary for 3.4.22.41 extracted from

  • Wang, B.; Shi, G.P.; Yao, P.M.; Li, Z.; Chapman, H.A.; Brömme, D.
    Human cathepsin F. Molecular cloning, functional expression, tissue localization, and enzymatic characterization (1998), J. Biol. Chem., 273, 32000-32008.
    View publication on PubMed
Search for more literature for 3.4.22.41

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
morpholine urea-Leu-homophenylalaninevinyl sulfone-phenyl potent and irreversible inhibitor of cysteine proteases Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00023
-
 N-benzyloxycarbonyl-L-Leu-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens
0.00044
-
 N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens
0.00124
-
 N-benzyloxycarbonyl-L-Val-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens
0.00287
-
 N-benzyloxycarbonyl-L-Arg-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 composed of a 88 residue propeptide and a 214 residue mature enzyme, no signal peptide Homo sapiens
23590
-
 mature enzyme, calculation from sequence of cDNA Homo sapiens
33860
-
 302 amino acid protein composed of a 88 residue propeptide and a 214 residue mature enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBX1 accession number AF071748, AF071749
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein four putative N-glycosylation site, one in the propeptide at Asn13, three in the mature region at Asn185, Asn196, Asn258 , no evidence of usage for in vivo glycosylation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-benzyloxycarbonyl-L-Arg-L-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens N-benzyloxycarbonyl-L-Arg-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-L-Leu-L-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens N-benzyloxycarbonyl-L-Leu-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens N-benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
 ?
N-benzyloxycarbonyl-L-Val-L-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens N-benzyloxycarbonyl-L-Val-L-Arg + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 N-benzyloxycarbonyl-L-Arg-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens
1.3
-
 N-benzyloxycarbonyl-L-Leu-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens
1.3
-
 N-benzyloxycarbonyl-L-Val-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens
2.5
-
 N-benzyloxycarbonyl-L-Phe-L-Arg-4-methylcoumarin 7-amide
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 6.8
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 4 h stable Homo sapiens
7.2
-
 half-life 2 min Homo sapiens