Literature summary for 3.4.22.40 extracted from

Broemme, D.; Rossi, A.B.; Smeekens, S.P.; Anderson, D.C.; Payan, D.G.
Human bleomycin hydrolase: molecular cloning, sequencing, functional expression, and enzymatic characterization (1996), Biochemistry, 35, 6706-6714.

Search for more literature for 3.4.22.40

Application

Application	Comment	Organism
medicine	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Sf9/baculovirus cells	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
1-(L-trans-epoxysuccinylleucylamino)-4-guanidinobutane	-	Homo sapiens
iodoacetate	-	Homo sapiens
NEM	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	6 * 52000, SDS-PAGE	Homo sapiens
310000	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
815	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
bleomycin + H2O	bleomycin B2	Homo sapiens	deamido-bleomycin	-	?
bleomycin + H2O	bleomycin A2	Homo sapiens	deamido-bleomycin	-	?
citrulline-4-methylcoumarin 7-amide + H2O	-	Homo sapiens	citrulline + 7-amino-4-methylcoumarin	-	?
Glu-4-methylcoumarin 7-amide + H2O	-	Homo sapiens	Glu + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 52000, SDS-PAGE	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	-	Homo sapiens

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8	-	Homo sapiens

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Release 2023.1 (January 2023)