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Literature summary for 3.4.22.40 extracted from

  • Mistou, M.Y.; Gripon, J.C.
    Catalytic properties of the cysteine aminopeptidase PepC, a bacterial bleomycin hydrolase (1998), Biochim. Biophys. Acta, 1383, 63-70.
    View publication on PubMed
Search for more literature for 3.4.22.40

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 Gly-Gly-Gly-Ala
-
 Lactococcus lactis
0.2
-
 Gly-Gly-Phe-Leu
-
 Lactococcus lactis
0.29
-
 Gly-Gly-Phe
-
 Lactococcus lactis
0.46
-
 bleomycin A2
-
 Lactococcus lactis
0.5
-
 Met-Ala-Ser
-
 Lactococcus lactis
0.57
-
 Gly-Gly-Leu
-
 Lactococcus lactis
0.63
-
 Thr-Ser-Lys
-
 Lactococcus lactis
0.66
-
 Gly-Gly-Ala
-
 Lactococcus lactis
0.75
-
 Gly-Gly-Arg
-
 Lactococcus lactis
0.78
-
 Gly-Gly-Gly-Gly
-
 Lactococcus lactis
1.01
-
 Gly-Gly-D-Leu
-
 Lactococcus lactis
1.05
-
 Gly-Gly-Gly
-
 Lactococcus lactis
1.05
-
 Gly-Gly-Tyr
-
 Lactococcus lactis
1.14
-
 Gly-Gly-Glu
-
 Lactococcus lactis
1.24
-
 Gly-Gly-Gly-Gly
-
 Lactococcus lactis
1.7
-
 Gly-Phe
-
 Lactococcus lactis
2.7
-
 Thr-Ser
-
 Lactococcus lactis
2.9
-
 Gly-Ala
-
 Lactococcus lactis
3.5
-
 Phe-p-nitroanilide
-
 Lactococcus lactis
4.1
-
 Gly-Gly-NH2
-
 Lactococcus lactis
4.6
-
 Gly-Leu
-
 Lactococcus lactis
4.7
-
 Gly-Lys
-
 Lactococcus lactis
5.6
-
 Gly-D-Leu
-
 Lactococcus lactis
6.1
-
 Arg-p-nitroanilide
-
 Lactococcus lactis
7
-
 Met-paranitroanilide
-
 Lactococcus lactis
9.5
-
 Met-Ala
-
 Lactococcus lactis
9.7
-
 Gly-p-nitroanilide
-
 Lactococcus lactis
9.9
-
 Lys-p-nitroanilide
-
 Lactococcus lactis
10.1
-
 Gly-Gly
-
 Lactococcus lactis
12.5
-
 Leu-p-nitroanilide
-
 Lactococcus lactis
13.5
-
 Gly-NH2
-
 Lactococcus lactis
14.8
-
 Gly-Asp
-
 Lactococcus lactis
17.4
-
 Ala-p-nitroanilide
-
 Lactococcus lactis
28.4
-
 Asp-p-nitroanilide
-
 Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-p-nitroanilide + H2O
-
 Lactococcus lactis Ala + 4-nitroaniline
-
 ?
Arg-p-nitroanilide + H2O
-
 Lactococcus lactis Arg + 4-nitroaniline
-
 ?
Asp-p-nitroanilide + H2O
-
 Lactococcus lactis Asp + 4-nitroaniline
-
 ?
bleomycin + H2O bleomycin A2 Lactococcus lactis deamido-bleomycin
-
 ?
bleomycin A2 + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Ala + H2O
-
 Lactococcus lactis Gly + Ala
-
 ?
Gly-Asp + H2O
-
 Lactococcus lactis Gly + Asp
-
 ?
Gly-D-Leu + H2O
-
 Lactococcus lactis Gly + D-Leu
-
 ?
Gly-Gly + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Ala + H2O
-
 Lactococcus lactis glycyl-L-alanine + glycine
-
 ?
Gly-Gly-Arg + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-D-Leu + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Glu + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Gly + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Gly-Ala + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Gly-Gly + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Gly-Gly-Gly + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Leu + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-NH2 + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Phe + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Phe-Leu + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Gly-Tyr + H2O
-
 Lactococcus lactis ?
-
 ?
Gly-Leu + H2O
-
 Lactococcus lactis Gly + Leu
-
 ?
Gly-Lys + H2O
-
 Lactococcus lactis Gly + Lys
-
 ?
Gly-NH2 + H2O
-
 Lactococcus lactis Gly + NH3
-
 ?
Gly-p-nitroanilide + H2O
-
 Lactococcus lactis Gly + 4-nitroaniline
-
 ?
Gly-Phe + H2O
-
 Lactococcus lactis Gly + Phe
-
 ?
Leu-p-nitroanilide + H2O
-
 Lactococcus lactis Leu + 4-nitroaniline
-
 ?
Lys-p-nitroanilide + H2O
-
 Lactococcus lactis Lys + 4-nitroaniline
-
 ?
Met-Ala + H2O
-
 Lactococcus lactis Met + Ala
-
 ?
Met-Ala-Ser + H2O
-
 Lactococcus lactis ?
-
 ?
Met-p-itroanilide + H2O
-
 Lactococcus lactis Met + 4-nitroaniline
-
 ?
Phe-p-nitroanilide + H2O
-
 Lactococcus lactis Phe + 4-nitroaniline
-
 ?
Thr-Ser + H2O
-
 Lactococcus lactis Thr + Ser
-
 ?
Thr-Ser-Lys + H2O
-
 Lactococcus lactis ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Lactococcus lactis

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Lactococcus lactis