Literature summary for 3.4.22.38 extracted from

Lecaille, F.; Broemme, D.; Lalmanach, G.
Biochemical properties and regulation of cathepsin K activity (2008), Biochimie, 90, 208-226.

Search for more literature for 3.4.22.38

Application

Application	Comment	Organism
medicine	cathepsin K is shown to play an essential role in rheumatoid arthritis, osteoarthritis, atherosclerosis, lung inflammation, amyloidosis, gaucher disease and obesity	Homo sapiens
medicine	one genetic disorder is traced to cathepsin K: peridontopathia. Osteoblasts from these patients accumulate undigested collagen fibrils. Nonsense, missense and stop codon mutations have been shown in patients	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
the overall organization of the catalytic site, which consists of two domains folds together to give a V-shaped active site cleft configuration. The central helix is the most prominent feature of the left domain, whereas the right domain is mostly dominated by beta-barrel motifs (5-6 strands). The active site lies at the interface between the two domains	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
Y67L/L205A	10fold lower interaction with inhibitor stefin B (Ki: 0.0000000008) suggesting that Tyr67 and Leu205 are critical for interaction with stefin B. Inhibitor cystatin C Ki: 0.0000000087, inhibitor L-kininogen Ki: 0.0000000135, inhibitor H-kininogen Ki: 0.0000000145	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
cystatin C	natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor	Homo sapiens
H-kininogen	natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor	Homo sapiens
L-kininogen	natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor	Homo sapiens
Stefin B	natural inhibitor of cathepsin K, reversible, tight-binding competitive inhibitor	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	one of the two putative N-linked glycosylation sites is located in the propeptide and the other is in the mature protease	Homo sapiens
additional information	protein is synthesized as a pre-proprotein	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Abz-His-Pro-Gly-Gly-Pro-Gln-EDDnp + H2O	cathepsin k is unusual among the cysteine cathepsins since it can cleave substrates with Pro in the P2 position. A specific peptide substrate for cathepsin K is developed (kcat/Km: 426000/Msec) that is resistant to proteolysis by cathepsin B, L, S, F, H,V and the serine proteases (cathepsin G, chymotrypsin and leukocyte elastase)	Homo sapiens	?	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
cathepsin K	-	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0000000008	-	Stefin B	Y67L/L205A mutant cathepsin K	Homo sapiens
0.0000000041	-	cystatin C	-	Homo sapiens
0.0000000049	-	L-kininogen	-	Homo sapiens
0.0000000054	-	H-kininogen	-	Homo sapiens
0.0000000087	-	cystatin C	Y67L/L205A mutant cathepsin K	Homo sapiens
0.0000000124	-	Stefin B	wild-type cathepsin K	Homo sapiens
0.0000000135	-	L-kininogen	Y67L/L205A mutant cathepsin K	Homo sapiens
0.0000000145	-	H-kininogen	Y67L/L205A mutant cathepsin K	Homo sapiens

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Release 2023.1 (January 2023)