Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycyl-glycine | increases the substrate hydrolysis, increases Km and kcat value | Porphyromonas gingivalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzamidine | - |
Porphyromonas gingivalis | |
benzamidine derivatives | overview, derivatives with an urea moiety linking the 2 aromatic rings, and derivatives with a less polar ether linker, the latter being less efficient inhibitors | Porphyromonas gingivalis | |
bis-benzamidine with urea linker | best inhibitor | Porphyromonas gingivalis | |
Zn2+ | complete inhibition of both enzyme forms at 6.7 mM, increases inhibitory effect of benzamidine derivatives 2-3fold, lowers Ki-values, binding mechanism | Porphyromonas gingivalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Porphyromonas gingivalis | - |
peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
purified enzyme forms HRgpA and RgpB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | - |
Porphyromonas gingivalis | peptides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arginine gingipain | - |
Porphyromonas gingivalis |
HRgpA | - |
Porphyromonas gingivalis |
RgpB | - |
Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Porphyromonas gingivalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki-values for benzamidine derivatives in presence or absence of Zn2+ | Porphyromonas gingivalis | |
0.013 | - |
bis-benzamidine with urea linker | pH 7.6, RgpB, in presence of Zn2+ | Porphyromonas gingivalis | |
0.015 | - |
bis-benzamidine with urea linker | pH 7.6, HRgpA, in presence of Zn2+ | Porphyromonas gingivalis | |
0.03 | - |
bis-benzamidine with urea linker | pH 7.6, HRgpA and RgpB, in absence of Zn2+ | Porphyromonas gingivalis |