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Literature summary for 3.4.22.36 extracted from

  • Fassy, F.; Krebs, O.; Rey, H.; Komara, B.; Gillard, C.; Capdevila, C.; Yea, C.; Faucheu, C.; Blanchet, A.M.; Miossec, C.; Diu-Hercend, A.
    Enzymic activity of two caspases related to interleukin-1beta-converting enzyme (1998), Eur. J. Biochem., 253, 76-83.
    View publication on PubMed
Search for more literature for 3.4.22.36

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baculovirus infected insect cells as 30000 Da proteins lacking the propeptide, automaturation into p20 and p10 subunits Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-DEVD-aldehyde
-
 Homo sapiens
acetyl-DEVS-aldehyde
-
 Homo sapiens
acetyl-YVAD-aldehyde
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 succinyl-YVAD-4-nitroanilide pH 7.5, 37°C Homo sapiens
0.02
-
 succinyl-YVAD-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the production of the active enzyme induces the activation of an endogenous 32000 Da (CPP32) like caspase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant T7-tagged enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-WEHD-7-amido-4-methylcoumarin + H2O WEHD is the optimal tetrapeptide recognition motif Homo sapiens acetyl-WEHD + 7-amino-4-methylcoumarin
-
 ?
additional information the production of the active enzyme induces the activation of an endogenous 32000 Da (CPP32) like caspase Homo sapiens ?
-
 ?
pre-interleukin-1beta + H2O
-
 Homo sapiens 17000 Da fragment + 28000 Da fragment
-
 ?
succinyl-YVAD-4-nitroanilide + H2O
-
 Homo sapiens succinyl-YVAD + 4-nitroaniline
-
 ?
succinyl-YVAD-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens succinyl-YVAD + 7-amino-4-methylcoumarin
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.35
-
 succinyl-YVAD-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens
1
-
 succinyl-YVAD-4-nitroanilide pH 7.5, 37°C Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
 acetyl-YVAD-aldehyde pH 7.5, 37°C Homo sapiens
0.00002
-
 acetyl-DEVS-aldehyde pH 7.5, 37°C Homo sapiens