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Literature summary for 3.4.22.35 extracted from
- Two cysteine protease inhibitors, EhICP1 and 2, localized in distinct compartments, negatively regulate secretion in Entamoeba histolytica (2006), FEBS Lett., 580, 5306-5312.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as His-tag fusion protein in Escherichia coli BL21(DE3), formation of inclusion bodies | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EhICP1 | Entamoeba histolytica inhibitor for cysteine protease type 1; Entamoeba histolytica inhibitor for cysteine protease type 1; Entamoeba histolytica inhibitor for cysteine protease type 1, inhibition also by regulation of CP5 processing | Entamoeba histolytica | |
| EhICP2 | Entamoeba histolytica inhibitor for cysteine protease type 2; Entamoeba histolytica inhibitor for cysteine protease type 2; Entamoeba histolytica inhibitor for cysteine protease type 2, inhibition also by regulation of CP5 processing | Entamoeba histolytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Entamoeba histolytica | 5764 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Entamoeba histolytica | essential for pathogenicity of Entamoeba histolytica | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
| Entamoeba histolytica | Q01957 | - |
- |
| Entamoeba histolytica | Q01958 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein using His-tag under denaturating conditions | Entamoeba histolytica |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| dialysis against refolding buffer | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| additional information | essential for pathogenicity of Entamoeba histolytica | Entamoeba histolytica | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CP1 | - |
Entamoeba histolytica |
| CP2 | - |
Entamoeba histolytica |
| CP5 | - |
Entamoeba histolytica |
| cysteine protease 1 | - |
Entamoeba histolytica |
| cysteine protease 2 | - |
Entamoeba histolytica |
| cysteine protease 5 | - |
Entamoeba histolytica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00000005 | - |
EhICP2 | pH 5.0, 37°C | Entamoeba histolytica | |
| 0.00000013 | - |
EhICP1 | pH 5.0, 37°C | Entamoeba histolytica | |
| 0.00000017 | - |
EhICP2 | pH 5.0, 37°C | Entamoeba histolytica | |
| 0.00000059 | - |
EhICP1 | pH 5.0, 37°C | Entamoeba histolytica | |
| 0.00000081 | - |
EhICP1 | pH 5.0, 37°C | Entamoeba histolytica | |
| 0.00000095 | - |
EhICP2 | pH 5.0, 37°C | Entamoeba histolytica |
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