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Literature summary for 3.4.22.35 extracted from

  • Scholze, H.; Tannich, E.
    Histolysain and other Entamoeba cysteine endopeptidases (2004), Handbook of Proteolytic Enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds), 2, 1164-1166.
No PubMed abstract available
Search for more literature for 3.4.22.35

Cloned(Commentary)

Cloned (Comment) Organism
expression of partial gene ehcp1 in Escherichia coli as GST-fusion protein, expression of functional EhCP2 in insect cells using the baculovirus transfection system Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface the enzyme is secreted and localized on the cell surface Entamoeba histolytica 9986
-
lysosome lysosome-like vesicles Entamoeba histolytica 5764
-
membrane the enzyme contains a transmembrane segment Entamoeba histolytica 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 1 * 27000, about Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Entamoeba histolytica the enzyme degrades a number of proteins, including those of the extracellular matrix ?
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba dispar
-
 gene edcp2 or acp2
-
Entamoeba histolytica
-
 gene ehcp2 or acp2 encodes histolysain, gene ehcp1 or acp1 encodes amoebapain
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no N-glycosylation Entamoeba histolytica

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
additional information the enzyme is expressed and active in different life cycle stages Entamoeba histolytica
-
trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Gly-Phe-Phe + H2O substrate of amoebapain Entamoeba histolytica Arg-Gly + Phe-Phe
-
 ?
azocasein + H2O
-
 Entamoeba histolytica ?
-
 ?
Azocoll + H2O
-
 Entamoeba histolytica ?
-
 ?
additional information the enzyme degrades a number of proteins, including those of the extracellular matrix Entamoeba histolytica ?
-
 ?
additional information the enzyme degrades a number of proteins, including those of the extracellular matrix, but also acts on small molecule substrates showing endopeptidase and exopeptidase-like activities, amoebapain hydrolyzes unblocked tetrapeptides with basic residues at P2 Entamoeba histolytica ?
-
 ?
Z-Arg-Arg-4-methoxy-2-nitroanilide + H2O
-
 Entamoeba histolytica Z-Arg-Arg + 4-methoxy-2-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 27000, about Entamoeba histolytica
More the enzyme contains an RGD integrin attachment motif, structure-function overview Entamoeba histolytica

Synonyms

Synonyms Comment Organism
amoebapain
-
 Entamoeba histolytica
EhCP1
-
 Entamoeba histolytica
EhCP2
-
 Entamoeba histolytica
Histolysin
-
 Entamoeba histolytica
Histolysin
-
 Entamoeba dispar
More the enzyme belongs to the C1 peptidase family Entamoeba histolytica
More the enzyme belongs to the C1 peptidase family Entamoeba dispar

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 6 dependent on the substrate Entamoeba histolytica

pI Value

Organism Comment pI Value Maximum pI Value
Entamoeba histolytica
-
-
 additional information