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Literature summary for 3.4.22.35 extracted from

  • Ocadiz, R.; Orozco, E.; Carrillo, E.; Quintas, L.I.; Ortega-Lopez, J.; Garcia-Perez, R.M.; Sanchez, T.; Castillo-Juarez, B.A.; Garcia-Rivera, G.; Rodriguez, M.A.
    EhCP112 is an Entamoeba histolytica secreted cysteine protease that may be involved in the parasite-virulence (2005), Cell. Microbiol., 7, 221-232.
    View publication on PubMed
Search for more literature for 3.4.22.35

Cloned(Commentary)

Cloned (Comment) Organism
gene ehcp112, functional expression of the His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, in Escherichia coli strain BL21 Entamoeba histolytica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular EhCP112 is secreted Entamoeba histolytica
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 x * 52000, recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, SDS-PAGE Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
collagen type 1 + H2O Entamoeba histolytica
-
 ?
-
 ?
Fibronectin + H2O Entamoeba histolytica
-
 ?
-
 ?
additional information Entamoeba histolytica the enzyme is involved in host tissue invasion by trophozoites and destroys cell monolayers ?
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
 gene ehcp112
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, from Escherichia coli strain BL21 by nickel affinity chromatography Entamoeba histolytica

Source Tissue

Source Tissue Comment Organism Textmining
trophozoite
-
 Entamoeba histolytica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Entamoeba histolytica ?
-
 ?
collagen type 1 + H2O
-
 Entamoeba histolytica ?
-
 ?
Fibronectin + H2O
-
 Entamoeba histolytica ?
-
 ?
additional information the enzyme is involved in host tissue invasion by trophozoites and destroys cell monolayers Entamoeba histolytica ?
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, recombinant His-tagged full-length EhCP112 zymogen, containing the signal peptide and the pro-peptide, SDS-PAGE Entamoeba histolytica
More the enzyme contains an RDG domain Entamoeba histolytica

Synonyms

Synonyms Comment Organism
EhCP112
-
 Entamoeba histolytica