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Literature summary for 3.4.22.35 extracted from

  • Hellberg, A.; Nowak, N.; Leippe, M.; Tannich, E.; Bruchhaus, I.
    Recombinant expression and purification of an enzymatically active cysteine proteinase of the protozoan parasite Entamoeba histolytica (2002), Protein Expr. Purif., 24, 131-137.
    View publication on PubMed
Search for more literature for 3.4.22.35

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged inactive pro-enzyme in Escherichia coli BL21(DE3) Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
antipain
-
 Entamoeba histolytica
iodoacetamide
-
 Entamoeba histolytica
leupeptin
-
 Entamoeba histolytica
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane ie.e. E-64, irreversible Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Entamoeba histolytica
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information processing of the inactive pro-enzyme to the mature active form by treatment with 10 mM DTT and 0.04% SDS at 37°C and pH 8.8, SDS can be substituted by Triton X-100 and octylglucoside but these are 3fold less efficient Entamoeba histolytica

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged inactive pro-enzyme to homogeneity Entamoeba histolytica

Renatured (Commentary)

Renatured (Comment) Organism
renaturation of purified inactive pro-enzyme by a 100fold dilution in a buffer containing reduced and oxidized thiols leading to a soluble inactive pro-enzyme Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
170
-
 purified native enzyme, substrate azocasein Entamoeba histolytica
180
-
 purified recombinant enzyme, substrate azocasein Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Entamoeba histolytica fragments of azocasein
-
 ?
benzyloxycarbonyl-Ala-Arg-Arg-4-nitroanilide + H2O
-
 Entamoeba histolytica benzyloxycarbonyl-Ala-Arg-Arg + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O
-
 Entamoeba histolytica benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
 ?
Bovine serum albumin + H2O
-
 Entamoeba histolytica ?
-
 ?
Collagen + H2O
-
 Entamoeba histolytica ?
-
 ?
Fibronectin + H2O
-
 Entamoeba histolytica ?
-
 ?
Gelatin + H2O
-
 Entamoeba histolytica ?
-
 ?
Hemoglobin + H2O
-
 Entamoeba histolytica ?
-
 ?
immunoglobulin G + H2O
-
 Entamoeba histolytica ?
-
 ?
additional information no activity with benzyloxycarbonyl-Phe-Arg-4-nitroanilide Entamoeba histolytica ?
-
 ?
mucin + H2O
-
 Entamoeba histolytica ?
-
 ?
protein + H2O
-
 Entamoeba histolytica peptides
-
 ?

Subunits

Subunits Comment Organism
? x * 27000-29000, recombinant and native enzyme, reducing and nonreducing SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
EhCP5
-
 Entamoeba histolytica
Entamoeba histolytica cysteine proteinase 5
-
 Entamoeba histolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 8 recombinant enzyme Entamoeba histolytica

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5 recombinant enzyme Entamoeba histolytica