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Literature summary for 3.4.22.35 extracted from
- Entamoeba histolytica: a novel cysteine protease and an adhesin form the 112kDa surface protein (1999), Mol. Microbiol., 33, 556-568.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Ehcp112, gene is encoded adjacent to Ehadh112, the latter encoding an adhesin that is covalently connected to the enzyme by electrostatic forces, DNA sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli BL21(DE3) | Entamoeba histolytica |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| phagosome | - |
Entamoeba histolytica | - |
- |
| plasma membrane | integral | Entamoeba histolytica | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 112000, enzyme attached to adhesin, reducing and nonreducing SDS-PAGE, x * 75000, about, enzyme without adhesin, SDS-PAGE | Entamoeba histolytica |
| 112000 | - |
x * 112000, enzyme attached to adhesin, reducing and nonreducing SDS-PAGE, x * 75000, about, enzyme without adhesin, SDS-PAGE | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protein + H2O | Entamoeba histolytica | - |
peptides | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
gene Ehcp112, gene is encoded adjacent to Ehadh112 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli | Entamoeba histolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| phagocyte | - |
Entamoeba histolytica | - |
| trophozoite | - |
Entamoeba histolytica | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is covalently connected to an adhesin by electrostatic forces which are not broken during phagocytosis | Entamoeba histolytica | ? | - |
? | |
| protein + H2O | - |
Entamoeba histolytica | peptides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 112000, enzyme attached to adhesin, reducing and nonreducing SDS-PAGE, x * 75000, about, enzyme without adhesin, SDS-PAGE | Entamoeba histolytica |
| More | enzyme contains a transmembranal segment, amino acid residues 259-280, and an RGD motif | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EhCP112 | - |
Entamoeba histolytica |
| Entamoeba histolytica 112kDa adhesin | together with a protein with an adherence domain EhADH112 | Entamoeba histolytica |
| Entamoeba histolytica cysteine protease | - |
Entamoeba histolytica |
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Release 2023.1 (January 2023)
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