Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Liposomes | association increases activity about 2fold | Entamoeba histolytica |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Entamoeba histolytica | |
p-hydroxymercuribenzoate | - |
Entamoeba histolytica | |
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane | i.e. E64 | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | bound to the host cell surface | Entamoeba histolytica | - |
- |
membrane | associated by hydrophobic interaction | Entamoeba histolytica | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protein + H2O | Entamoeba histolytica | enzyme is involved in pathogenic destruction of host tissue by degradation of extracellular matrix proteins | peptides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | Q95030 | enzyme is termed CP5 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity | Entamoeba histolytica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
trophozoite | - |
Entamoeba histolytica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
470 | - |
purified enzyme | Entamoeba histolytica |
890 | - |
purified enzyme associated to liposomes | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-arginyl-L-arginine 4-nitroanilide + H2O | - |
Entamoeba histolytica | benzyloxycarbonyl-L-arginyl-L-arginine + 4-nitroaniline | - |
? | |
complement factor C3 + H2O | - |
Entamoeba histolytica | peptides | product determination | ? | |
complement factor C9 + H2O | - |
Entamoeba histolytica | peptides | product determination | ? | |
fibronectin + H2O | - |
Entamoeba histolytica | peptides | product determination | ? | |
immunoglobulin G + H2O | - |
Entamoeba histolytica | peptides derived from immunoglobulin G | product determination | ? | |
additional information | no activity with peptide substrates containing phenylalanine at P2 position instead of arginine | Entamoeba histolytica | ? | - |
? | |
protein + H2O | enzyme is involved in pathogenic destruction of host tissue by degradation of extracellular matrix proteins | Entamoeba histolytica | peptides | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure modeling | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
CP5 | - |
Entamoeba histolytica |
More | enzyme is a member of the cysteine proteinase family | Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | - |
Entamoeba histolytica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Entamoeba histolytica | - |
- |
8.12 |