Application | Comment | Organism |
---|---|---|
medicine | since recombinant legumain-1 needs trans-activation through a cathepsin, it is probably involved in an enzyme cascade and therefore remains interesting as a candidate in a multi-component vaccine | Dictyocaulus viviparus |
Cloned (Comment) | Organism |
---|---|
C-terminal hexa-His-tagged is expressed in the yeast Pichia pastoris | Dictyocaulus viviparus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cystatin | 0.1 mM, 90.5% remaining activity | Dictyocaulus viviparus | |
iodoacetic acid | 0.1 mM, 82.4% remaining activity | Dictyocaulus viviparus | |
additional information | no inhibition by the general cysteine protease inhibitor E-64 | Dictyocaulus viviparus | |
N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone | 0.1 mM, 0.23% remaining activity | Dictyocaulus viviparus | |
pepstatin A | 0.01 mM, 86.9% remaining activity | Dictyocaulus viviparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05195 | - |
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin | pH 5.5, 39°C | Dictyocaulus viviparus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48310 | - |
calculated from sequence | Dictyocaulus viviparus |
50000 | - |
MALDI-TOF mass spectrometry analysis | Dictyocaulus viviparus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyocaulus viviparus | I6R478 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | C-terminal hexa-His-tag is cleaved off together with the pro-peptide | Dictyocaulus viviparus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | - |
Dictyocaulus viviparus | - |
intestine | - |
Dictyocaulus viviparus | - |
testis | - |
Dictyocaulus viviparus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Dictyocaulus viviparus | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
bovine collagen types I + H2O | - |
Dictyocaulus viviparus | ? | - |
? | |
bovine collagen types II + H2O | - |
Dictyocaulus viviparus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LEG-1 | - |
Dictyocaulus viviparus |
legumain-1 | - |
Dictyocaulus viviparus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 39 | recombinant legumain-1 after trans-activation | Dictyocaulus viviparus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin | pH 5.5, 39°C | Dictyocaulus viviparus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant legumain-1 after trans-activation | Dictyocaulus viviparus |
General Information | Comment | Organism |
---|---|---|
metabolism | for sufficient functionality, recombinant legumain-1 needs trans-activation through Dictyocaulus viviparus cathepsin L-2. Since recombinant legumain-1 needs trans-activation through a cathepsin, it is probably involved in an enzyme cascade and therefore remains interesting as a candidate in a multi-component vaccine | Dictyocaulus viviparus |