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Literature summary for 3.4.22.34 extracted from

  • Holzhausen, J.; Haake, C.; Schicht, S.; Hinse, P.; Jordan, D.; Kremmer, E.; Strube, C.
    Biological function of Dictyocaulus viviparus asparaginyl peptidase legumain-1 and its suitability as a vaccine target (2018), Parasitology, 145, 378-392 .
    View publication on PubMed

Application

Application Comment Organism
medicine since recombinant legumain-1 needs trans-activation through a cathepsin, it is probably involved in an enzyme cascade and therefore remains interesting as a candidate in a multi-component vaccine Dictyocaulus viviparus

Cloned(Commentary)

Cloned (Comment) Organism
C-terminal hexa-His-tagged is expressed in the yeast Pichia pastoris Dictyocaulus viviparus

Inhibitors

Inhibitors Comment Organism Structure
cystatin 0.1 mM, 90.5% remaining activity Dictyocaulus viviparus
iodoacetic acid 0.1 mM, 82.4% remaining activity Dictyocaulus viviparus
additional information no inhibition by the general cysteine protease inhibitor E-64 Dictyocaulus viviparus
N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone 0.1 mM, 0.23% remaining activity Dictyocaulus viviparus
pepstatin A 0.01 mM, 86.9% remaining activity Dictyocaulus viviparus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05195
-
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin pH 5.5, 39°C Dictyocaulus viviparus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48310
-
calculated from sequence Dictyocaulus viviparus
50000
-
MALDI-TOF mass spectrometry analysis Dictyocaulus viviparus

Organism

Organism UniProt Comment Textmining
Dictyocaulus viviparus I6R478
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein C-terminal hexa-His-tag is cleaved off together with the pro-peptide Dictyocaulus viviparus

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
Dictyocaulus viviparus
-
intestine
-
Dictyocaulus viviparus
-
testis
-
Dictyocaulus viviparus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
Dictyocaulus viviparus benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
?
bovine collagen types I + H2O
-
Dictyocaulus viviparus ?
-
?
bovine collagen types II + H2O
-
Dictyocaulus viviparus ?
-
?

Synonyms

Synonyms Comment Organism
LEG-1
-
Dictyocaulus viviparus
legumain-1
-
Dictyocaulus viviparus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 39 recombinant legumain-1 after trans-activation Dictyocaulus viviparus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin pH 5.5, 39°C Dictyocaulus viviparus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant legumain-1 after trans-activation Dictyocaulus viviparus

General Information

General Information Comment Organism
metabolism for sufficient functionality, recombinant legumain-1 needs trans-activation through Dictyocaulus viviparus cathepsin L-2. Since recombinant legumain-1 needs trans-activation through a cathepsin, it is probably involved in an enzyme cascade and therefore remains interesting as a candidate in a multi-component vaccine Dictyocaulus viviparus