Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-Glu-Ser-Glu-Asn-aldehyde | hydrolysis of the the Asn-containing substrate inhibited in a concentration-dependent manner | Nicotiana benthamiana | |
acetyl-Glu-Ser-Glu-Asn-aldehyde | hydrolysis of the the Asn-containing substrate inhibited in a concentration-dependent manner | Oldenlandia affinis | |
acetyl-Glu-Ser-Glu-Asn-aldehyde | hydrolysis of the the Asn-containing substrate inhibited in a concentration-dependent manner | Viola tricolor | |
Acetyl-Tyr-Val-Ala-Asp-aldehyde | hydrolysis of the the Asn-containing substrate inhibited in a concentration-dependent manner, reduced amounts of cyclic cyclotide produced in transiently transformed tobacco leaves determined | Nicotiana benthamiana | |
Acetyl-Tyr-Val-Ala-Asp-aldehyde | hydrolysis of the the Asn-containing substrate inhibited in a concentration-dependent manner | Oldenlandia affinis | |
Acetyl-Tyr-Val-Ala-Asp-aldehyde | hydrolysis of the the Asn-containing substrate inhibited in a concentration-dependent manner | Viola tricolor | |
pepstatin A | no effect on ability to cleave the Asn-containing substrate | Nicotiana benthamiana | |
pepstatin A | no effect on ability to cleave the Asn-containing substrate | Oldenlandia affinis | |
pepstatin A | no effect on ability to cleave the Asn-containing substrate | Viola tricolor | |
phenylmethylsulfonyl fluoride | no effect on ability to cleave the Asn-containing substrate | Nicotiana benthamiana | |
phenylmethylsulfonyl fluoride | no effect on ability to cleave the Asn-containing substrate | Oldenlandia affinis | |
phenylmethylsulfonyl fluoride | no effect on ability to cleave the Asn-containing substrate | Viola tricolor | |
trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane | i.e. E-64, no effect on ability to cleave the Asn-containing substrate | Nicotiana benthamiana | |
trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane | i.e. E-64, no effect on ability to cleave the Asn-containing substrate | Oldenlandia affinis | |
trans-epoxysuccinyl-L-leucylamido(4-guanidino)butane | i.e. E-64, no effect on abilityto cleave the Asn-containing substrate | Viola tricolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Nicotiana benthamiana | role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event | ? | - |
? | |
additional information | Viola tricolor | role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event | ? | - |
? | |
additional information | Oldenlandia affinis | role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana benthamiana | - |
no endogenous cyclotides, transiently transformed with the cyclotide kalata B1 | - |
Oldenlandia affinis | - |
endogenous cyclotides as potential substrates for backbone cyclization by legumain analyzed | - |
Viola tricolor | - |
endogenous cyclotides as potential substrates for backbone cyclization by legumain analyzed | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | leaf extracts, assay for asparaginyl bond hydrolysis in | Viola tricolor | - |
leaf | leaf extracts, assay for asparaginyl bond hydrolysis in | Oldenlandia affinis | - |
leaf | leaf extracts, assay for asparaginyl bond hydrolysis in, analysis of backbone cyclization of endogenous cyclotides in | Nicotiana benthamiana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
involvement of legumain in backbone cyclization of plant-produced cyclotides analyzed, assay for asparaginyl bond hydrolysis in leaf extracts described | Viola tricolor |
additional information | - |
involvement of legumain in backbone cyclization of plant-produced cyclotides analyzed, assay for asparaginyl bond hydrolysis in leaf extracts described | Oldenlandia affinis |
additional information | - |
involvement of legumain in backbone cyclization of plant-produced cyclotides determined, transient expression of precursor of the cyclotide kalata B1 in tobacco leads to production of the circular cyclotide, in vivo asparaginyl bond hydrolysis shown to be necessary for cyclization, reduced amounts of cyclic cyclotide shown by suppression of legumain either by decreasing gene expression or by a specific inhibitor, detection and quantification of cyclotide proteins by MALDI-TOF | Nicotiana benthamiana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | role of legumain in backbone cyclization of proteins analyzed, catalysis of protein backbone cyclization by coupling asparaginyl bond hydrolysis at the C terminus of the cyclotide domain with peptide bond ligation | Viola tricolor | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | role of legumain in backbone cyclization of proteins analyzed, catalysis of protein backbone cyclization by coupling asparaginyl bond hydrolysis at the C terminus of the cyclotide domain with peptide bond ligation | Oldenlandia affinis | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | role of legumain in backbone cyclization of proteins determined, catalysis of protein backbone cyclization by coupling asparaginyl bond hydrolysis at the C terminus of the cyclotide domain with peptide bond ligation | Nicotiana benthamiana | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
additional information | role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event | Nicotiana benthamiana | ? | - |
? | |
additional information | role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event | Viola tricolor | ? | - |
? | |
additional information | role in backbone cyclization of proteins determined, catalyis of peptide bond cleavage and of ligation of plant-specific cyclotides in a single processing event | Oldenlandia affinis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AEP | - |
Nicotiana benthamiana |
AEP | - |
Viola tricolor |
AEP | - |
Oldenlandia affinis |
asparagine endopeptidase | - |
Nicotiana benthamiana |
asparagine endopeptidase | - |
Viola tricolor |
asparagine endopeptidase | - |
Oldenlandia affinis |