Application | Comment | Organism |
---|---|---|
biotechnology | studies on vacuolar targeting, targeting motif of legumain as a potential tool for plant engineering analyzed | Saccharum officinarum |
Cloned (Comment) | Organism |
---|---|
in Escherichia coli, construct pCvsEndoexp1 transformed into callus derived from Q117 meristematic tissue, transgenic plants regenerated | Saccharum officinarum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vacuole | vacuoles within the sugarcane storage parenchyma tissue, bioinformatical studies on vacuolar targeting, GFP-constructs sucessfully targeted to lytic vacuolar compartments of Saccharum officinarum | Saccharum officinarum | 5773 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharum officinarum | Q0ZHB0 | encoded by VPE-1 gene; sugarcane, transgenic plants regenerated from callus culture | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | C-terminus, in endogenous and in recombinant protein | Saccharum officinarum |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli, SDS-PAGE | Saccharum officinarum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | for generation of transformed plants, GFP reporter analysis indicated | Saccharum officinarum | - |
whole plant | control plants and transformed plants, GFP reporter analysis indicated | Saccharum officinarum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
cleavage sites for N- and C-terminus indicated, predicted motif for vacuolar proteins analyzed, targeting ability sucessfully tested in Saccharum officinarum and in other plant species by transient expression analysis after biolistic bombardment using the construct pCvsEndoexp1 | Saccharum officinarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | bioinformatic approach, studies on vacuolar targeting by determination of minimal vacuole targeting motifs, GFP reporter analysis and subcellular staining indicated | Saccharum officinarum | ? | - |
? | |
additional information | bioinformatic approach, studies on vacuolar targeting, determination of minimal vacuole targeting motifs, GFP reporter analysis and subcellular staining indicated | Saccharum officinarum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Asparaginyl endopeptidase | - |
Saccharum officinarum |