Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FaCPI-1 | strawberry cystatin, phytocystatin of Fragaria sp. | Hordeum vulgare | |
HvCPI-4 | cystatin HvCPI-4 of Hordeum vulgare | Hordeum vulgare | |
HvCPI-4-deltaT143-Cterm | C-terminal extended part of cystatin HvCPI-4 | Hordeum vulgare | |
HvCPI-4-deltaT151-Cterm | C-terminal extended part of cystatin HvCPI-4 | Hordeum vulgare | |
HvCPI-4-Q86P | cystatin HvCPI-4 of Hordeum vulgare, mutant Q86P | Hordeum vulgare | |
additional information | inhibitory activity of the phytocystatins HvCPI-4 of Hordeum vulgare and FaCPI-1 of Fragaria sp. measured by the inhibition of legumain proteinase activity, ability of HvCPI-4 and FaCPI-1 to inhibit human legumain in vitro demonstrated | Homo sapiens | |
additional information | inhibitory activity measured by the inhibition of legumain proteinase activity, phytocystatins that are able to inhibit human legumain also inhibit the barley legumain proteinase activities in developing roots, endogenous barley legumain activities inhibited by iodoacetamide, no inhibition by the non-legumain inhibitors leupeptin and E64 observed; inhibitory effects against endogenous legumain activity in barley protein extracts tested, inhibitory activity measured by the inhibition of legumain-like proteinase activity, phytocystatins able to inhibit human legumain also inhibit the barley legumain proteinase activities in developing endosperms, endogenous barley legumain activities inhibited by iodoacetamide, no inhibition by the non-legumain inhibitors leupeptin and E64 observed | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q99538 | Homo sapiens | - |
Hordeum vulgare | - |
legumain activity in barley protein extracts analyzed | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | inhibitory effects by phytocystatins against commercial human legumain analyzed | Homo sapiens | - |
endosperm | developing endosperms of 18 days after flowering, inhibitory effects by cystatins against endogenous legumain activity in barley protein extracts analyzed | Hordeum vulgare | - |
root | collected from plants germinated at 22°C in the dark for 7 days | Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
ability of carboxy-terminal extensions and mutants of phytocystatin to inhibit endogenous legumain activity present in barley protein extracts analyzed, inhibitory activity measured by the inhibition of legumain proteinase activity, assay described | Hordeum vulgare |
additional information | - |
ability of carboxy-terminal extensions and mutants of phytocystatin to inhibit in vitro human legumain shown, inhibitory activity measured by the inhibition of legumain proteinase activity, assay described | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | inhibitory activity of cystatin variants of Hordeum vulgare against endogenous legumain activity in protein extracts of Hordeum vulgare analyzed | Hordeum vulgare | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | inhibitory activity of cystatin variants of Hordeum vulgare against endogenous legumain activity in protein extracts of roots of Hordeum vulgare analyzed | Hordeum vulgare | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O | inhibitory activity of phytocystatin variants of Hordeum vulgare tested against commercial human legumain | Homo sapiens | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AEP | - |
Hordeum vulgare |
AEP | - |
Homo sapiens |
asparagine endopeptidase | - |
Hordeum vulgare |
asparagine endopeptidase | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
HvCPI-4-Q86P | mutant Q86P of HvCPI-4, inhibitor concentrations between 25 and 500 nM used, incubation with 10 microgram of a soluble endosperm protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00013 | - |
HvCPI-4-Q86P | mutant Q86P of HvCPI-4, inhibitor concentrations between 25 and 500 nM used, incubation with 5 microgram of a soluble root protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00019 | - |
HvCPI-4 | inhibitor concentrations between 25 and 500 nM used, incubation with 10 microgram of a soluble endosperm protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00019 | - |
HvCPI-4-deltaT143-Cterm | inhibitor concentrations between 25 and 500 nM used, incubation with 10 microgram of a soluble endosperm protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00019 | - |
HvCPI-4 | inhibitor concentrations between 25 and 500 nM used, incubation with 5 microgram of a soluble root protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00019 | - |
HvCPI-4-deltaT143-Cterm | inhibitor concentrations between 25 and 500 nM used, incubation with 5 microgram of a soluble root protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00029 | - |
HvCPI-4-deltaT151-Cterm | inhibitor concentrations between 25 and 500 nM used, incubation with 10 microgram of a soluble endosperm protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00029 | - |
HvCPI-4-deltaT151-Cterm | inhibitor concentrations between 25 and 500 nM used, incubation with 5 microgram of a soluble root protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00076 | - |
FaCPI-1 | inhibitor concentrations between 25 and 500 nM used, incubation with 10 microgram of a soluble endosperm protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare | |
0.00076 | - |
FaCPI-1 | inhibitor concentrations between 25 and 500 nM used, incubation with 5 microgram of a soluble root protein extract, at 25°C and pH 6 for 10 min | Hordeum vulgare |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
inhibitory doses for 50% proteinase activity inhibition higher for root legumain activity than for endosperm one | Hordeum vulgare | additional information | |
0.018 | - |
endosperm | Hordeum vulgare | HvCPI-4-deltaT143-Cterm | |
0.02 | - |
endosperm | Hordeum vulgare | FaCPI-1 | |
0.022 | - |
endosperm | Hordeum vulgare | HvCPI-4-deltaT151-Cterm | |
0.033 | - |
endosperm | Hordeum vulgare | HvCPI-4-Q86P | |
0.038 | - |
endosperm | Hordeum vulgare | HvCPI-4 | |
0.068 | - |
root extract | Hordeum vulgare | HvCPI-4-deltaT143-Cterm | |
0.091 | - |
root extract | Hordeum vulgare | HvCPI-4-deltaT151-Cterm | |
0.11 | - |
root extract | Hordeum vulgare | HvCPI-4-Q86P | |
0.12 | - |
root extract | Hordeum vulgare | HvCPI-4 | |
0.2 | - |
root extract | Hordeum vulgare | FaCPI-1 |