Any feedback?
Literature summary for 3.4.22.34 extracted from
- Schistosome legumain (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. )Academic Press, 2, 1305-1310.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form | Schistosoma mansoni |
| expression of C197 in Pichia pastoris, expression as beta-galactosidase fusion protein in Escherichia coli, the recombinant enzyme shows hemoglobinase activity, the N197 enzyme form might not be the active one, but the C197 form | Schistosoma mansoni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| E64 | slight inhibition at 1 mM | Schistosoma mansoni |  |
| iodoacetamide | - |
Schistosoma mansoni | |
| leupeptin | slight inhibition at 1 mM | Schistosoma mansoni | |
| additional information | no inhibition by Z-Phe-Ala-CHN2 and 1,10-phenanthroline | Schistosoma mansoni | |
| N-ethylmaleimide | - |
Schistosoma mansoni | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted into the digestive tract of host animals | Schistosoma mansoni | - |
- |
| extracellular | the enzyme is secreted into the digestive tract of host animals | Schistosoma japonicum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect by DTT | Schistosoma mansoni |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
| 32000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
| 32000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
| 50000 | - |
x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
| 50000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
| 50000 | - |
x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hemoglobin + H2O | Schistosoma mansoni | the enzyme is involved in host hemoglobin degradation | ? | - |
? | |
| Hemoglobin + H2O | Schistosoma japonicum | the enzyme is involved in host hemoglobin degradation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q99538 | - |
- |
| Schistosoma japonicum | P42665 | the enzyme contains a Cys at position 197 | - |
| Schistosoma mansoni | P09841 | from infected mice and humans | - |
| Schistosoma mansoni | Q9NFY9 | fragment; from infected mice and humans | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | autoactivation | Schistosoma mansoni |
| proteolytic modification | autoactivation | Schistosoma japonicum |
| proteolytic modification | autoactivation at 37°C, pH 4.5 | Schistosoma mansoni |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme to homogeneity | Schistosoma mansoni |
| soluble recombinant C197 from Pichia pastoris, native enzyme to homogeneity | Schistosoma mansoni |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | active site His151 | Schistosoma mansoni | |
| hydrolysis of proteins and small molecule substrates at -Asn-/-Xaa- bonds | active site His151 and Cys197 | Schistosoma mansoni |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | activity only in adult worms | Schistosoma mansoni | - |
| additional information | activity only in adult worms | Schistosoma japonicum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Schistosoma mansoni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hemoglobin + H2O | - |
Schistosoma japonicum | ? | - |
? | |
| Hemoglobin + H2O | the enzyme is involved in host hemoglobin degradation | Schistosoma mansoni | ? | - |
? | |
| Hemoglobin + H2O | the enzyme is involved in host hemoglobin degradation | Schistosoma japonicum | ? | - |
? | |
| Hemoglobin + H2O | the recombinant beta-galactosidase fusion enzyme shows hemoglobinase activity | Schistosoma mansoni | ? | - |
? | |
| additional information | the enzyme strictly requires Asn at P1, and prefers Ala, Thr, Val, Asn in descending order at P2, and Thr, Ala, Val, and Ile at P3, overview | Schistosoma mansoni | ? | - |
? | |
| Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
| Z-Ala-Pro-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Pro-Asn + 7-amino-4-methylcoumarin | - |
? | |
| Z-Ala-Ser-Asn-7-amido-4-methylcoumarin + H2O | - |
Schistosoma mansoni | Z-Ala-Ser-Asn + 7-amino-4-methylcoumarin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma japonicum |
| ? | x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation | Schistosoma mansoni |
| ? | x * 32000, SDS-PAGE, x * 50000, amino acid sequence calculation of the proenzyme | Schistosoma mansoni |
| More | structure analysis, overview | Schistosoma mansoni |
| More | structure analysis, overview | Schistosoma japonicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Antigen Sj32 | - |
Schistosoma japonicum |
| Antigen SM32 | - |
Schistosoma mansoni |
| C197 legumain | - |
Schistosoma mansoni |
| c197 Sm32 | - |
Schistosoma mansoni |
| cysteine protease 1 | - |
Homo sapiens |
| hemoglobinase | - |
Schistosoma mansoni |
| hemoglobinase | - |
Schistosoma japonicum |
| LGMN | - |
Homo sapiens |
| More | the enzyme belongs to the C13 peptidase family | Homo sapiens |
| More | the enzyme belongs to the C13 peptidase family | Schistosoma mansoni |
| More | the enzyme belongs to the C13 peptidase family | Schistosoma japonicum |
| N197 legumain | - |
Schistosoma mansoni |
| schistosome legumain | - |
Schistosoma mansoni |
| schistosome legumain | - |
Schistosoma japonicum |
| Sj32 | - |
Schistosoma japonicum |
| Sm32 | - |
Schistosoma mansoni |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 45 | - |
Schistosoma mansoni |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Schistosoma mansoni |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.8 | recombinant enzyme | Schistosoma mansoni |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
