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Literature summary for 3.4.22.34 extracted from

  • Chen, J.M.; Dando, P.M.; Rawlings, N.D.; Brown, M.A.; Young, N.E.; Stevens, R.A.; Hewitt, E.; Watts, C.; Barrett, A.J.
    Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase (1997), J. Biol. Chem., 272, 8090-8098.
    View publication on PubMed
Search for more literature for 3.4.22.34

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination and analysis Homo sapiens
partial DNA sequence determination Sus scrofa

General Stability

General Stability Organism
enzyme tends to be absorbed to any solid material at low salt concentration Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Human cystatin C
-
 Sus scrofa
iodoacetamide
-
 Sus scrofa
iodoacetate
-
 Sus scrofa
additional information no inhibition by E-64, i.e. trans-epoxysuccinyl-L-leucylamido-(4-guanidino)-butane, no inhibition by PMSF, pepstatin and 1,10-phenanthroline Sus scrofa
N-ethylmaleimide
-
 Sus scrofa
N-Phenylmaleimide
-
 Sus scrofa
ovocystatin from chicken egg white Sus scrofa
p-chloromercuribenzoate
-
 Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.8, 30°C Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE Sus scrofa
34000
-
 x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Sus scrofa
-
 peptides
-
 ?
protein + H2O Homo sapiens
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q99538
-
-
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 4 potential N-glycosylation sites at Asn91, ASn167, Asn263, and Asn272 Sus scrofa
glycoprotein 4 potential N-glycosylation sites at Asn91, ASn167, Asn263, and Asn272 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
2200fold from kidney, to homogeneity Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Sus scrofa
-
liver
-
 Sus scrofa
-
additional information no activity in blood leukocytes Homo sapiens
-
placenta low activity Homo sapiens
-
spleen
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.1
-
 purified enzyme, substrate benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide, pH 5.8, 30°C Sus scrofa

Storage Stability

Storage Stability Organism
4°C to -20°C, pH 5.8, 0.05 M sodium citrate, 0.2 M NaCl, 1 mM EDTA, 10 mm cysteine, 0.1% CHAPS, stable for several months with little loss of activity Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allatotropin + H2O
-
 Sus scrofa ?
-
 ?
azocasein + H2O
-
 Sus scrofa ?
-
 ?
benzoyl-Asn-4-nitroanilide + H2O
-
 Sus scrofa benzoyl-Asn + 4-nitroaniline
-
 ?
benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide + H2O
-
 Sus scrofa benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
bombesin + H2O
-
 Sus scrofa ?
-
 ?
chicken vasoactive intestinal peptide fragment 16-28 + H2O
-
 Sus scrofa ?
-
 ?
human vasoactive intestinal peptide fragment 1-12 + H2O
-
 Sus scrofa ?
-
 ?
additional information no activity with angiotensin I, [Asn1,Val5]angiotensin II, peptides ASTTNYT and GSNKGAIIGLM, substance P, and dynorphin A fragment 1-13, no activity with Asn-4-methylcoumarin-7-amide or Asp-4-methylcoumarin-7-amide Sus scrofa ?
-
 ?
neurotensin + H2O
-
 Sus scrofa ?
-
 ?
neurotensin fragment 1-11 + H2O
-
 Sus scrofa ?
-
 ?
pig vasoactive intestinal peptide fragment 1-28 + H2O
-
 Sus scrofa ?
-
 ?
protein + H2O
-
 Sus scrofa peptides
-
 ?
protein + H2O
-
 Homo sapiens peptides
-
 ?
recombinant C-fragment of tetanus toxoid + H2O cleavage of 3 peptide bonds Sus scrofa ?
-
 ?
somatostatin + H2O
-
 Sus scrofa ?
-
 ?

Subunits

Subunits Comment Organism
? x * 34000, glycosylated enzyme, SDS-PAGE, x * 31000, deglycosylated enzyme, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
asparaginyl endopepidase
-
 Sus scrofa
Asparaginyl endopeptidase
-
 Homo sapiens
More belongs to the peptidase family C13 Sus scrofa
More belongs to the peptidase family C13 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
46
-
 benzyloxycarbonyl-Ala-Ala-Asn-4-methylcoumarin-7-amide pH 5.8, 30°C Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 at 30°C Sus scrofa
6.4
-
 at 25°C Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
3 6
-
 Sus scrofa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000005
-
 Human cystatin C below, pH 5.8, 30°C Sus scrofa