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Literature summary for 3.4.22.33 extracted from
- Kinetics studies with fruit bromelain (Ananas comosus) in the presence of cysteine and divalent ions (2015), J. Food Sci. Technol., 52, 5954-5960 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | noncompetitive inhibition at pH 3.5, while both K-and V-type activations of bromelain are observed in the presence of 0.5 mM Ca2+ at pH 4.5 and pH 7.5 | Ananas comosus |  |
| Cu2+ | competitive inhibition at pH 3.5 with 0.6 mM Cu2+, which changes to an uncompetitive inhibition at pH 4.5 and pH 7.5 | Ananas comosus | |
| Hg2+ | uncompetitive inhibition at pH 3.5 and pH 4.5 with 0.6 mM Hg2+ | Ananas comosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Ananas comosus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | noncompetitive inhibition at pH 3.5, while both K-and V-type activations of bromelain are observed in the presence of 0.5 mM Ca2+ at pH 4.5 and pH 7.5 | Ananas comosus | |
| additional information | modulation of fruit bromelain by divalent ions is pH dependent | Ananas comosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | O23791 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 7.23fold from fruits by ammonium sulfate fractionation, dialysis, and anion exchange chromatography | Ananas comosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | - |
Ananas comosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2512.44 | - |
purified native enzyme, pH and temperature not specified in the publication | Ananas comosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Gelatin + H2O | - |
Ananas comosus | ? | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics, detailed overview | Ananas comosus |
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Release 2023.1 (January 2023)
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