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Literature summary for 3.4.22.33 extracted from

  • Sao Paulo Barretto Miranda, I.K.; Fontes Suzart Miranda, A.; Souza, F.V.; Vannier-Santos, M.A.; Pirovani, C.P.; Pepe, I.M.; Rodowanski, I.J.; Ferreira, K.T.; Mendes Souza Vaz, L.; de Assis, S.A.
    The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells (2017), Int. J. Food Sci. Nutr., 68, 442-454 .
    View publication on PubMed
Search for more literature for 3.4.22.33

Application

Application Comment Organism
pharmacology bromelain is pharmacologically active against B16F10 melanoma cells with complete inhibition of tumor cell proliferation in vitro Ananas comosus

Inhibitors

Inhibitors Comment Organism Structure
additional information stability of bromelain in the presence of EDTA and sodium benzoate; stability of bromelain in the presence of EDTA and sodium benzoate Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus O23791 pulp enzyme; an unexplored French Guiana variety, collected in 1993
-
Ananas comosus O23801 peel enzyme; an unexplored French Guiana variety, collected in 1993
-
Ananas comosus AGB 772 O23791 pulp enzyme; an unexplored French Guiana variety, collected in 1993
-
Ananas comosus AGB 772 O23801 peel enzyme; an unexplored French Guiana variety, collected in 1993
-

Source Tissue

Source Tissue Comment Organism Textmining
exocarp
-
 Ananas comosus
-
additional information analysis of bromelain activity in different Ananas comosus parts and tissues, overview Ananas comosus
-
plant crown
-
 Ananas comosus
-
pulp
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1050.57
-
 extracted crown bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus
1365.77
-
 extracted peel bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus
1519.85
-
 extracted pulp bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Ananas comosus ?
-
 ?
casein + H2O
-
 Ananas comosus AGB 772 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 60 pulp enzyme Ananas comosus
50 70 crown enzyme Ananas comosus
60 80 peel enzyme Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Ananas comosus

General Information

General Information Comment Organism
physiological function antiproliferative effect of bromelain from different tissues against B16F10 murine melanoma cells, overview Ananas comosus