BRENDA - Enzyme Database
show all sequences of 3.4.22.33

Preliminary functional characterization, cloning and primary sequence of Fastuosain, a cysteine peptidase isolated from fruits of Bromelia fastuosa

Cabral, H.; Leopoldino, A.M.; Tajara, E.H.; Greene, L.J.; Faca, V.M.; Mateus, R.P.; Ceron, C.R.; de Souza Judice, W.A.; Julianod, L.; Bonilla-Rodriguez, G.O.; Protein Pept. Lett. 13, 83-89 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
highest activity at 96 mM, at 37°C and pH 7.0
Bromelia fastuosa
dithiothreitol
highest activity at 2.5 mM, at pH 7.0 and 37°C
Bromelia fastuosa
EDTA
28% increase of activity at 2 mM
Bromelia fastuosa
Urea
activity decreases in the presence of 4 M urea and reaches the activity of the control again at 8 M when using casein as a substrate
Bromelia fastuosa
Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli Top10 cells
Bromelia fastuosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
12% residual activity at 0.16 mM
Bromelia fastuosa
cystatin
-
Bromelia fastuosa
Hg2+
14% residual activity at 0.16 mM
Bromelia fastuosa
iodoacetic acid
5% residual activity at 2 mM
Bromelia fastuosa
additional information
not inhibited by pepstatin
Bromelia fastuosa
p-chloromercuribenzoate
15% residual activity at 2 mM
Bromelia fastuosa
Phenylmethylsulfonylfluoride
84% residual activity at 2 mM
Bromelia fastuosa
Urea
at 2.4 M urea the activity decreases continuously to 50% when using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
SDS-PAGE
Bromelia fastuosa
Organism
Organism
UniProt
Commentary
Textmining
Bromelia fastuosa
Q6Q2T4
fragment
-
Purification (Commentary)
Purification (Commentary)
Organism
CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration
Bromelia fastuosa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Bromelia fastuosa
-
leaf
-
Bromelia fastuosa
-
stem
-
Bromelia fastuosa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
benzyloxyxarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
casein + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Ala-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Cys(S-Bzl)-Leu-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Gly-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Pro-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
N-alpha-benzoyl-DL-arginine-2-naphthylamide + H2O
-
682716
Bromelia fastuosa
N-alpha-benzoyl-DL-arginine + 2-naphthylamine
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
fastuosain
-
Bromelia fastuosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
using casein as a subatrate
Bromelia fastuosa
65
-
using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Bromelia fastuosa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
9
-
Bromelia fastuosa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
highest activity at 96 mM, at 37°C and pH 7.0
Bromelia fastuosa
dithiothreitol
highest activity at 2.5 mM, at pH 7.0 and 37°C
Bromelia fastuosa
EDTA
28% increase of activity at 2 mM
Bromelia fastuosa
Urea
activity decreases in the presence of 4 M urea and reaches the activity of the control again at 8 M when using casein as a substrate
Bromelia fastuosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Top10 cells
Bromelia fastuosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
12% residual activity at 0.16 mM
Bromelia fastuosa
cystatin
-
Bromelia fastuosa
Hg2+
14% residual activity at 0.16 mM
Bromelia fastuosa
iodoacetic acid
5% residual activity at 2 mM
Bromelia fastuosa
additional information
not inhibited by pepstatin
Bromelia fastuosa
p-chloromercuribenzoate
15% residual activity at 2 mM
Bromelia fastuosa
Phenylmethylsulfonylfluoride
84% residual activity at 2 mM
Bromelia fastuosa
Urea
at 2.4 M urea the activity decreases continuously to 50% when using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
SDS-PAGE
Bromelia fastuosa
Purification (Commentary) (protein specific)
Commentary
Organism
CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration
Bromelia fastuosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Bromelia fastuosa
-
leaf
-
Bromelia fastuosa
-
stem
-
Bromelia fastuosa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
benzyloxyxarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
casein + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Ala-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Cys(S-Bzl)-Leu-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Gly-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Pro-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
N-alpha-benzoyl-DL-arginine-2-naphthylamide + H2O
-
682716
Bromelia fastuosa
N-alpha-benzoyl-DL-arginine + 2-naphthylamine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
using casein as a subatrate
Bromelia fastuosa
65
-
using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Bromelia fastuosa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
9
-
Bromelia fastuosa
Other publictions for EC 3.4.22.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752632
Misran
-
Properties of bromelain extra ...
Ananas comosus
Biocatal. Agricult. Biotechnol.
18
101095
2019
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
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-
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-
-
-
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-
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-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
754265
Ghensi
In vitro effect of bromelain ...
Ananas comosus
J. Craniofac. Surg.
30
1064-1067
2019
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
-
-
1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
754304
Zhi
-
Effect of high pressure proce ...
Ananas comosus
J. Food Process Eng.
42
e13146
2019
1
1
-
-
-
-
-
-
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1
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1
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1
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1
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2
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1
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1
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1
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2
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-
-
-
-
-
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-
753631
Ramli
Comparative structural analys ...
Ananas comosus
Food Chem.
266
183-191
2018
-
1
-
-
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-
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2
-
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1
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2
2
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1
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1
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-
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-
1
-
-
2
2
-
-
-
-
-
-
-
1
-
1
1
-
-
-
753642
Meza-Espinoza
Enzyme activity and partial c ...
Bromelia karatas, Bromelia pinguin
Food Sci. Biotechnol.
27
509-517
2018
2
-
-
-
-
-
5
2
-
-
4
-
-
2
-
-
2
-
-
2
4
-
6
-
-
2
-
8
-
2
2
-
-
-
-
-
2
-
-
-
-
-
-
-
5
-
2
-
-
4
-
-
-
-
2
-
2
4
-
6
-
2
-
8
-
2
2
-
-
-
-
-
-
-
-
754274
Andjaitan
-
Bromelain enzyme from pineapp ...
Ananas comosus
J. Eng. Appl. Sci.
13
3125-3130
2018
-
-
-
-
-
-
-
-
-
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1
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3
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3
-
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-
-
-
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-
-
-
-
-
1
1
-
-
-
753890
Sao Paulo Barretto Miranda
The biochemical characterizat ...
Ananas comosus, Ananas comosus AGB 772
Int. J. Food Sci. Nutr.
68
442-454
2017
-
1
-
-
-
-
1
-
-
-
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-
4
-
-
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4
3
-
4
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3
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1
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2
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2
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-
5
3
-
4
-
3
-
-
-
2
-
-
-
-
1
2
-
-
-
754238
Das
Destabilization of human insu ...
Ananas comosus
J. Cell. Biochem.
118
4881-4896
2017
-
-
-
-
-
-
-
-
-
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-
4
-
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1
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-
3
-
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1
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1
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1
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1
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3
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1
-
1
-
-
-
1
-
-
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-
1
1
-
-
-
752429
Mohan
-
Optimisation of bromelain enz ...
Ananas comosus
Am. J. Biochem. Biotechnol.
12
188-195
2016
-
1
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-
2
-
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1
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2
1
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1
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1
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1
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2
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2
1
-
1
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
753812
Chakraborty
-
Modeling the inactivation kin ...
Ananas comosus
Innov. Food Sci. Emerg. Technol.
33
10-18
2016
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
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1
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1
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1
1
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1
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1
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1
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1
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1
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1
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1
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-
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-
-
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-
754309
Kaur
Kinetics studies with fruit b ...
Ananas comosus
J. Food Sci. Technol.
52
5954-5960
2015
-
-
-
-
-
-
3
1
-
2
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1
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1
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1
1
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1
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1
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3
1
1
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2
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1
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1
1
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1
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-
-
-
-
-
-
731210
Arshad
Bromelain: an overview of indu ...
Ananas comosus
Appl. Microbiol. Biotechnol.
98
7283-7297
2014
-
-
-
-
-
-
-
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1
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2
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1
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5
1
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3
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1
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4
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1
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1
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1
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5
1
3
-
1
-
4
-
-
1
-
-
-
-
-
-
717050
Liang
-
Study on the stability of frui ...
Ananas comosus
Adv. Mater. Res.
421
19-22
2012
-
-
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2
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1
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1
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1
1
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2
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-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
731843
Corzo
-
Pineapple fruit bromelain affi ...
Ananas comosus
Food Chem.
133
631-635
2012
-
-
-
-
-
-
-
-
-
-
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1
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1
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5
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3
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4
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1
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5
-
3
-
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-
4
-
-
-
-
-
-
-
-
-
731908
Devi
-
Pineapple fruit bromelain and ...
Ananas comosus
Indian J. Agric. Biochem.
25
1-7
2012
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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-
1
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1
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1
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1
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1
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-
1
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-
-
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-
717487
Ferreira
-
Purification of bromelain from ...
Ananas comosus
Chem. Eng. Transact.
24
931-936
2011
-
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1
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1
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1
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1
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1
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1
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717625
Yin
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695660
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An inhibitive determination me ...
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665083
Rowan
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Fruit bromelain ...
Ananas comosus
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650803
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Bromelain: biochemistry, pharm ...
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Inhibition of cysteine protein ...
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Bromelain enzymes ...
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30301
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Immunochemical studies on ping ...
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Sasaki
Antigenic determinant common t ...
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