BRENDA - Enzyme Database show
show all sequences of 3.4.22.33

Preliminary functional characterization, cloning and primary sequence of Fastuosain, a cysteine peptidase isolated from fruits of Bromelia fastuosa

Cabral, H.; Leopoldino, A.M.; Tajara, E.H.; Greene, L.J.; Faca, V.M.; Mateus, R.P.; Ceron, C.R.; de Souza Judice, W.A.; Julianod, L.; Bonilla-Rodriguez, G.O.; Protein Pept. Lett. 13, 83-89 (2006)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
highest activity at 96 mM, at 37C and pH 7.0
Bromelia fastuosa
dithiothreitol
highest activity at 2.5 mM, at pH 7.0 and 37C
Bromelia fastuosa
EDTA
28% increase of activity at 2 mM
Bromelia fastuosa
Urea
activity decreases in the presence of 4 M urea and reaches the activity of the control again at 8 M when using casein as a substrate
Bromelia fastuosa
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli Top10 cells
Bromelia fastuosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
12% residual activity at 0.16 mM
Bromelia fastuosa
cystatin
-
Bromelia fastuosa
Hg2+
14% residual activity at 0.16 mM
Bromelia fastuosa
iodoacetic acid
5% residual activity at 2 mM
Bromelia fastuosa
additional information
not inhibited by pepstatin
Bromelia fastuosa
p-chloromercuribenzoate
15% residual activity at 2 mM
Bromelia fastuosa
Phenylmethylsulfonylfluoride
84% residual activity at 2 mM
Bromelia fastuosa
Urea
at 2.4 M urea the activity decreases continuously to 50% when using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
SDS-PAGE
Bromelia fastuosa
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bromelia fastuosa
Q6Q2T4
fragment
-
Purification (Commentary)
Commentary
Organism
CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration
Bromelia fastuosa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Bromelia fastuosa
-
leaf
-
Bromelia fastuosa
-
stem
-
Bromelia fastuosa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzyloxyxarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
casein + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Ala-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Cys(S-Bzl)-Leu-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Gly-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Pro-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
N-alpha-benzoyl-DL-arginine-2-naphthylamide + H2O
-
682716
Bromelia fastuosa
N-alpha-benzoyl-DL-arginine + 2-naphthylamine
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
using casein as a subatrate
Bromelia fastuosa
65
-
using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Bromelia fastuosa
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4
9
-
Bromelia fastuosa
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
highest activity at 96 mM, at 37C and pH 7.0
Bromelia fastuosa
dithiothreitol
highest activity at 2.5 mM, at pH 7.0 and 37C
Bromelia fastuosa
EDTA
28% increase of activity at 2 mM
Bromelia fastuosa
Urea
activity decreases in the presence of 4 M urea and reaches the activity of the control again at 8 M when using casein as a substrate
Bromelia fastuosa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli Top10 cells
Bromelia fastuosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
12% residual activity at 0.16 mM
Bromelia fastuosa
cystatin
-
Bromelia fastuosa
Hg2+
14% residual activity at 0.16 mM
Bromelia fastuosa
iodoacetic acid
5% residual activity at 2 mM
Bromelia fastuosa
additional information
not inhibited by pepstatin
Bromelia fastuosa
p-chloromercuribenzoate
15% residual activity at 2 mM
Bromelia fastuosa
Phenylmethylsulfonylfluoride
84% residual activity at 2 mM
Bromelia fastuosa
Urea
at 2.4 M urea the activity decreases continuously to 50% when using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
25000
-
SDS-PAGE
Bromelia fastuosa
Purification (Commentary) (protein specific)
Commentary
Organism
CM-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephadex G-50 gel filtration
Bromelia fastuosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Bromelia fastuosa
-
leaf
-
Bromelia fastuosa
-
stem
-
Bromelia fastuosa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzyloxyxarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
casein + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Ala-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Cys(S-Bzl)-Leu-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Leu-Gly-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
epsilon-NH2-Cap-Pro-Cys(S-Bzl)-7-amido-4-methylcoumarin + H2O
-
682716
Bromelia fastuosa
?
-
-
-
?
N-alpha-benzoyl-DL-arginine-2-naphthylamide + H2O
-
682716
Bromelia fastuosa
N-alpha-benzoyl-DL-arginine + 2-naphthylamine
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
-
using casein as a subatrate
Bromelia fastuosa
65
-
using N-alpha-benzoyl-DL-arginine-2-naphthylamide as a substrate
Bromelia fastuosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Bromelia fastuosa
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4
9
-
Bromelia fastuosa
Other publictions for EC 3.4.22.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731210
Arshad
Bromelain: an overview of indu ...
Ananas comosus
Appl. Microbiol. Biotechnol.
98
7283-7297
2014
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
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-
5
1
3
-
1
-
4
-
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-
-
1
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1
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1
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5
1
3
-
1
-
4
-
-
1
-
-
-
-
-
-
717050
Liang
-
Study on the stability of frui ...
Ananas comosus
Adv. Mater. Res.
421
19-22
2012
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-
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-
-
-
2
-
-
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1
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1
1
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2
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-
1
1
-
-
-
-
-
-
-
-
-
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-
731843
Corzo
-
Pineapple fruit bromelain affi ...
Ananas comosus
Food Chem.
133
631-635
2012
-
-
-
-
-
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1
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1
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5
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3
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4
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731908
Devi
-
Pineapple fruit bromelain and ...
Ananas comosus
Indian J. Agric. Biochem.
25
1-7
2012
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1
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1
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1
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1
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1
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1
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717487
Ferreira
-
Purification of bromelain from ...
Ananas comosus
Chem. Eng. Transact.
24
931-936
2011
-
-
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1
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1
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1
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717625
Yin
Preparative purification of br ...
Ananas comosus
Food Chem.
129
925-932
2011
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
-
1
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-
1
-
-
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-
-
-
-
-
-
718411
Gautam
-
Comparative study of extractio ...
Ananas comosus
Thai J. Pharm. Sci.
34
67-76
2010
-
-
-
-
-
-
-
-
-
-
1
-
-
1
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1
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1
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1
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1
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-
1
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1
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
710318
Neuteboom
An extended AE-rich N-terminal ...
Ananas comosus
Plant Physiol.
151
515-527
2009
-
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1
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3
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1
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2
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695660
Shukor
An inhibitive determination me ...
Ananas comosus
Appl. Biochem. Biotechnol.
144
283-291
2008
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2
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1
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6
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6
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681047
Hemavathi
-
Reverse micellar extraction of ...
Ananas comosus
J. Chem. Technol. Biotechnol.
82
985-992
2007
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1
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1
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-
682716
Cabral
Preliminary functional charact ...
Bromelia fastuosa
Protein Pept. Lett.
13
83-89
2006
4
-
1
-
-
-
8
-
-
-
1
-
-
3
-
-
1
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-
5
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9
-
2
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1
1
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4
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1
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8
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1
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1
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5
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9
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2
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1
1
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665208
Hale
Proteinase activity and stabil ...
Ananas comosus
Int. Immunopharmacol.
5
783-793
2005
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2
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1
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665083
Rowan
-
Fruit bromelain ...
Ananas comosus
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1137-1138
2004
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1
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1
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650803
Maurer
Bromelain: biochemistry, pharm ...
Ananas comosus
Cell. Mol. Life Sci.
58
1234-1245
2001
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2
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1
2
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1
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1
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1
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1
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5
1
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1
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1
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-
30296
Suh
-
Purification and characterizat ...
Ananas comosus
Han'guk Nonghwa Hakhoechi
35
300-307
1992
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2
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2
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1
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1
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1
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2
1
1
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2
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2
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1
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1
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2
1
1
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30279
Rowan
Inhibition of cysteine protein ...
Ananas comosus
FEBS Lett.
269
328-330
1990
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1
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4
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30278
Ota
Reinvestigation of fractionati ...
Ananas comosus
J. Biochem.
98
219-228
1985
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1
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2
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1
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1
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1
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9
1
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4
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-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
1
-
-
9
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
30274
Murachi
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
45
475-485
1976
-
-
-
-
-
-
1
2
-
-
-
-
-
1
-
-
1
-
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
1
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
30299
Yamada
Purification and characterizat ...
Ananas comosus
J. Biochem.
79
1223-1234
1976
-
-
-
-
-
-
-
2
-
-
1
-
-
2
-
1
1
-
-
2
-
-
6
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
1
-
2
-
-
6
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
30301
Toro-Goyco
Immunochemical studies on ping ...
Ananas comosus, Bromelia pinguin
Arch. Biochem. Biophys.
175
359-366
1976
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30300
Sasaki
Antigenic determinant common t ...
Ananas comosus
J. Biochem.
74
635-637
1973
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30277
Ota
Fractionation and some propert ...
Ananas comosus
J. Biochem.
71
817-830
1972
-
-
-
-
-
-
-
2
-
-
3
-
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
1
-
1
-
-
2
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
30273
Murachi
-
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
19
273-284
1970
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
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-