BRENDA - Enzyme Database
show all sequences of 3.4.22.33

Preparative purification of bromelain (EC 3.4.22.33) from pineapple fruit by high-speed counter-current chromatography using a reverse-micelle solvent system

Yin, L.; Sun, C.; Han, X.; Xu, L.; Xu, Y.; Qi, Y.; Peng, J.; Food Chem. 129, 925-932 (2011)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
SDS-PAGE
Ananas comosus
Organism
Organism
UniProt
Commentary
Textmining
Ananas comosus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit
Ananas comosus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
casein + H2O
-
717625
Ananas comosus
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Bromelain
-
Ananas comosus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Ananas comosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Ananas comosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
34000
-
SDS-PAGE
Ananas comosus
Purification (Commentary) (protein specific)
Commentary
Organism
high-speed counter-current chromatography coupled with the reverse micelle solvent system consisting of 0.10 g/ml cetyltrimethylammonium bromide (CTAB)/isooctane-hexylalcohol and 0.05 M sodium phosphate buffer is successfully applied to separate bromelain from pineapple fruit
Ananas comosus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
casein + H2O
-
717625
Ananas comosus
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Ananas comosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Ananas comosus
Other publictions for EC 3.4.22.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
752632
Misran
-
Properties of bromelain extra ...
Ananas comosus
Biocatal. Agricult. Biotechnol.
18
101095
2019
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1
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754265
Ghensi
In vitro effect of bromelain ...
Ananas comosus
J. Craniofac. Surg.
30
1064-1067
2019
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1
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1
1
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754304
Zhi
-
Effect of high pressure proce ...
Ananas comosus
J. Food Process Eng.
42
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2019
1
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753631
Ramli
Comparative structural analys ...
Ananas comosus
Food Chem.
266
183-191
2018
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2
2
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1
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1
1
-
-
-
753642
Meza-Espinoza
Enzyme activity and partial c ...
Bromelia karatas, Bromelia pinguin
Food Sci. Biotechnol.
27
509-517
2018
2
-
-
-
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5
2
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-
4
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2
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2
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2
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5
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2
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4
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2
4
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6
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2
-
8
-
2
2
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-
-
754274
Andjaitan
-
Bromelain enzyme from pineapp ...
Ananas comosus
J. Eng. Appl. Sci.
13
3125-3130
2018
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1
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1
1
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753890
Sao Paulo Barretto Miranda
The biochemical characterizat ...
Ananas comosus, Ananas comosus AGB 772
Int. J. Food Sci. Nutr.
68
442-454
2017
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1
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4
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3
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2
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5
3
-
4
-
3
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2
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1
2
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-
-
754238
Das
Destabilization of human insu ...
Ananas comosus
J. Cell. Biochem.
118
4881-4896
2017
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4
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1
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3
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1
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1
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1
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1
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1
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1
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1
1
-
-
-
752429
Mohan
-
Optimisation of bromelain enz ...
Ananas comosus
Am. J. Biochem. Biotechnol.
12
188-195
2016
-
1
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2
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2
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2
1
-
1
-
-
-
-
-
1
1
-
-
-
1
1
-
-
-
753812
Chakraborty
-
Modeling the inactivation kin ...
Ananas comosus
Innov. Food Sci. Emerg. Technol.
33
10-18
2016
-
-
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1
-
1
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1
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1
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1
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1
1
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1
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1
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1
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-
754309
Kaur
Kinetics studies with fruit b ...
Ananas comosus
J. Food Sci. Technol.
52
5954-5960
2015
-
-
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3
1
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1
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1
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1
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1
1
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1
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731210
Arshad
Bromelain: an overview of indu ...
Ananas comosus
Appl. Microbiol. Biotechnol.
98
7283-7297
2014
-
-
-
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1
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2
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1
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1
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1
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4
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1
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1
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1
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5
1
3
-
1
-
4
-
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1
-
-
-
-
-
-
717050
Liang
-
Study on the stability of frui ...
Ananas comosus
Adv. Mater. Res.
421
19-22
2012
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2
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1
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1
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1
1
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2
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-
-
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1
1
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731843
Corzo
-
Pineapple fruit bromelain affi ...
Ananas comosus
Food Chem.
133
631-635
2012
-
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1
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1
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1
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5
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3
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4
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731908
Devi
-
Pineapple fruit bromelain and ...
Ananas comosus
Indian J. Agric. Biochem.
25
1-7
2012
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1
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1
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717487
Ferreira
-
Purification of bromelain from ...
Ananas comosus
Chem. Eng. Transact.
24
931-936
2011
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717625
Yin
Preparative purification of br ...
Ananas comosus
Food Chem.
129
925-932
2011
-
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1
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1
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718411
Gautam
-
Comparative study of extractio ...
Ananas comosus
Thai J. Pharm. Sci.
34
67-76
2010
-
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1
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1
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1
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710318
Neuteboom
An extended AE-rich N-terminal ...
Ananas comosus
Plant Physiol.
151
515-527
2009
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1
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695660
Shukor
An inhibitive determination me ...
Ananas comosus
Appl. Biochem. Biotechnol.
144
283-291
2008
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2
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1
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2
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6
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2
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6
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681047
Hemavathi
-
Reverse micellar extraction of ...
Ananas comosus
J. Chem. Technol. Biotechnol.
82
985-992
2007
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1
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682716
Cabral
Preliminary functional charact ...
Bromelia fastuosa
Protein Pept. Lett.
13
83-89
2006
4
-
1
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8
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1
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3
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1
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5
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9
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1
2
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1
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4
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1
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8
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1
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5
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9
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2
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1
1
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665208
Hale
Proteinase activity and stabil ...
Ananas comosus
Int. Immunopharmacol.
5
783-793
2005
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2
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665083
Rowan
-
Fruit bromelain ...
Ananas comosus
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1137-1138
2004
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650803
Maurer
Bromelain: biochemistry, pharm ...
Ananas comosus
Cell. Mol. Life Sci.
58
1234-1245
2001
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2
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5
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30296
Suh
-
Purification and characterizat ...
Ananas comosus
Han'guk Nonghwa Hakhoechi
35
300-307
1992
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30279
Rowan
Inhibition of cysteine protein ...
Ananas comosus
FEBS Lett.
269
328-330
1990
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4
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30278
Ota
Reinvestigation of fractionati ...
Ananas comosus
J. Biochem.
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219-228
1985
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9
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4
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9
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4
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30274
Murachi
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
45
475-485
1976
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1
2
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5
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30299
Yamada
Purification and characterizat ...
Ananas comosus
J. Biochem.
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1223-1234
1976
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6
1
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6
1
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30301
Toro-Goyco
Immunochemical studies on ping ...
Ananas comosus, Bromelia pinguin
Arch. Biochem. Biophys.
175
359-366
1976
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30300
Sasaki
Antigenic determinant common t ...
Ananas comosus
J. Biochem.
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635-637
1973
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1
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30277
Ota
Fractionation and some propert ...
Ananas comosus
J. Biochem.
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817-830
1972
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2
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3
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30273
Murachi
-
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
19
273-284
1970
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