BRENDA - Enzyme Database
show all sequences of 3.4.22.33

Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains

Ota, S.; Muta, E.; Katahira, Y.; Okamoto, Y.; J. Biochem. 98, 219-228 (1985)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
scarcely inhibited by chicken chystatin
Ananas comosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE
Ananas comosus
27000
-
x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE
Ananas comosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ananas comosus
-
-
-
Purification (Commentary)
Commentary
Organism
2 forms: FBA and FBB; fractionation
Ananas comosus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
fruit
-
Ananas comosus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Benzoyl-Gly-Gly-Lys + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
?
-
-
-
-
Benzyloxycarbonyl-Ala-Leu + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Benzyloxycarbonyl-Ala + Leu
-
-
-
-
Benzyloxycarbonyl-Gly-Leu + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Leu
-
-
-
-
Benzyloxycarbonyl-Gly-Phe + H2O
-
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Phe
-
-
-
-
Benzyloxycarbonyl-Gly-Tyr + H2O
-
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Tyr
-
-
-
-
Benzyloxycarbonyl-Gly-Val + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Val
-
-
-
-
Carbobenzoxy-Gly-Ala + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Carbobenzoxy-Gly + Ala
-
-
-
-
additional information
specificity overview
30278
Ananas comosus
?
-
-
-
-
Nalpha-Benzoyl-Arg amide + H2O
-
30278
Ananas comosus
Nalpha-Benzoyl-Arg + NH3
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE
Ananas comosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
4
benzoyl-Glu-Tyr, benzoyl-Glu-Phe
Ananas comosus
3.5
-
benzoyl-Ala-Leu, benzoyl-Ala-Phe, enzyme form FBA
Ananas comosus
4
-
benzoyl-Ala-Leu, enzyme form FBA
Ananas comosus
6.5
-
benzoyl-Arg amide, enzyme form FBA
Ananas comosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
scarcely inhibited by chicken chystatin
Ananas comosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
23000
-
x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE
Ananas comosus
27000
-
x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE
Ananas comosus
Purification (Commentary) (protein specific)
Commentary
Organism
2 forms: FBA and FBB; fractionation
Ananas comosus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
fruit
-
Ananas comosus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Benzoyl-Gly-Gly-Lys + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
?
-
-
-
-
Benzyloxycarbonyl-Ala-Leu + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Benzyloxycarbonyl-Ala + Leu
-
-
-
-
Benzyloxycarbonyl-Gly-Leu + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Leu
-
-
-
-
Benzyloxycarbonyl-Gly-Phe + H2O
-
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Phe
-
-
-
-
Benzyloxycarbonyl-Gly-Tyr + H2O
-
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Tyr
-
-
-
-
Benzyloxycarbonyl-Gly-Val + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Benzyloxycarbonyl-Gly + Val
-
-
-
-
Carbobenzoxy-Gly-Ala + H2O
enzyme form FBA is much more active than FBB
30278
Ananas comosus
Carbobenzoxy-Gly + Ala
-
-
-
-
additional information
specificity overview
30278
Ananas comosus
?
-
-
-
-
Nalpha-Benzoyl-Arg amide + H2O
-
30278
Ananas comosus
Nalpha-Benzoyl-Arg + NH3
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 23000, Ananas comosus, enzyme form FBA, SDS-PAGE, x * 27000, Ananas comosus, enzyme form FBB, SDS-PAGE
Ananas comosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5
4
benzoyl-Glu-Tyr, benzoyl-Glu-Phe
Ananas comosus
3.5
-
benzoyl-Ala-Leu, benzoyl-Ala-Phe, enzyme form FBA
Ananas comosus
4
-
benzoyl-Ala-Leu, enzyme form FBA
Ananas comosus
6.5
-
benzoyl-Arg amide, enzyme form FBA
Ananas comosus
Other publictions for EC 3.4.22.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731210
Arshad
Bromelain: an overview of indu ...
Ananas comosus
Appl. Microbiol. Biotechnol.
98
7283-7297
2014
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-
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-
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1
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2
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1
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5
1
3
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1
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4
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1
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1
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1
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5
1
3
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1
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4
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1
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-
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717050
Liang
-
Study on the stability of frui ...
Ananas comosus
Adv. Mater. Res.
421
19-22
2012
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2
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1
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1
1
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2
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1
1
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731843
Corzo
-
Pineapple fruit bromelain affi ...
Ananas comosus
Food Chem.
133
631-635
2012
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-
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1
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1
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5
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3
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4
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1
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5
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3
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4
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731908
Devi
-
Pineapple fruit bromelain and ...
Ananas comosus
Indian J. Agric. Biochem.
25
1-7
2012
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1
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1
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1
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1
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717487
Ferreira
-
Purification of bromelain from ...
Ananas comosus
Chem. Eng. Transact.
24
931-936
2011
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1
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1
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1
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717625
Yin
Preparative purification of br ...
Ananas comosus
Food Chem.
129
925-932
2011
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1
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1
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1
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1
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718411
Gautam
-
Comparative study of extractio ...
Ananas comosus
Thai J. Pharm. Sci.
34
67-76
2010
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1
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1
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1
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1
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1
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1
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1
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1
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1
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710318
Neuteboom
An extended AE-rich N-terminal ...
Ananas comosus
Plant Physiol.
151
515-527
2009
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1
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3
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1
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2
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1
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1
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2
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695660
Shukor
An inhibitive determination me ...
Ananas comosus
Appl. Biochem. Biotechnol.
144
283-291
2008
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2
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1
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2
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2
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6
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2
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1
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2
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6
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681047
Hemavathi
-
Reverse micellar extraction of ...
Ananas comosus
J. Chem. Technol. Biotechnol.
82
985-992
2007
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1
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1
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682716
Cabral
Preliminary functional charact ...
Bromelia fastuosa
Protein Pept. Lett.
13
83-89
2006
4
-
1
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-
8
-
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1
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3
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1
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5
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9
-
2
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1
1
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4
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1
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8
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1
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1
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5
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9
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2
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1
1
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665208
Hale
Proteinase activity and stabil ...
Ananas comosus
Int. Immunopharmacol.
5
783-793
2005
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2
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1
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1
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2
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1
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665083
Rowan
-
Fruit bromelain ...
Ananas comosus
Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press
2
1137-1138
2004
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1
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1
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2
1
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1
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650803
Maurer
Bromelain: biochemistry, pharm ...
Ananas comosus
Cell. Mol. Life Sci.
58
1234-1245
2001
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2
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1
2
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1
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1
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3
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5
1
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1
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1
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2
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1
2
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1
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5
1
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1
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1
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30296
Suh
-
Purification and characterizat ...
Ananas comosus
Han'guk Nonghwa Hakhoechi
35
300-307
1992
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2
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2
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1
1
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30279
Rowan
Inhibition of cysteine protein ...
Ananas comosus
FEBS Lett.
269
328-330
1990
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30278
Ota
Reinvestigation of fractionati ...
Ananas comosus
J. Biochem.
98
219-228
1985
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1
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2
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1
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1
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1
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9
1
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4
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1
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1
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1
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9
1
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4
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30274
Murachi
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
45
475-485
1976
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1
2
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1
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1
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5
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1
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1
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2
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1
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1
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5
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1
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30299
Yamada
Purification and characterizat ...
Ananas comosus
J. Biochem.
79
1223-1234
1976
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2
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1
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2
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1
1
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2
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6
1
1
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2
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2
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1
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1
1
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2
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6
1
1
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2
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30301
Toro-Goyco
Immunochemical studies on ping ...
Ananas comosus, Bromelia pinguin
Arch. Biochem. Biophys.
175
359-366
1976
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2
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30300
Sasaki
Antigenic determinant common t ...
Ananas comosus
J. Biochem.
74
635-637
1973
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1
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1
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1
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30277
Ota
Fractionation and some propert ...
Ananas comosus
J. Biochem.
71
817-830
1972
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2
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3
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1
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1
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1
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2
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3
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2
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3
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1
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1
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2
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3
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30273
Murachi
-
Bromelain enzymes ...
Ananas comosus
Methods Enzymol.
19
273-284
1970
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1
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1
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1
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1
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1
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1
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3
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1
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