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Literature summary for 3.4.22.32 extracted from

  • Sao Paulo Barretto Miranda, I.K.; Fontes Suzart Miranda, A.; Souza, F.V.; Vannier-Santos, M.A.; Pirovani, C.P.; Pepe, I.M.; Rodowanski, I.J.; Ferreira, K.T.; Mendes Souza Vaz, L.; de Assis, S.A.
    The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells (2017), Int. J. Food Sci. Nutr., 68, 442-454 .
    View publication on PubMed
Search for more literature for 3.4.22.32

Application

Application Comment Organism
pharmacology bromelain is pharmacologically active against B16F10 melanoma cells with complete inhibition of tumor cell proliferation in vitro Ananas comosus

Inhibitors

Inhibitors Comment Organism Structure
additional information stability of bromelain in the presence of EDTA and sodium benzoate Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus P14518 an unexplored French Guiana variety, collected in 1993
-
Ananas comosus AGB 772 P14518 an unexplored French Guiana variety, collected in 1993
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Ananas comosus
-
additional information analysis of bromelain activity in different Ananas comosus parts and tissues, overview Ananas comosus
-
stem
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
432.98
-
 extracted stem bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus
859.44
-
 extracted leaf bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
casein + H2O
-
 Ananas comosus ?
-
 ?
casein + H2O
-
 Ananas comosus AGB 772 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 stem enzyme Ananas comosus
65
-
 leaf enzyme Ananas comosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 80 bromelain from the leaves shows higher activity between 60 and 80°C Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 leaf (first peak) and stem enzymes Ananas comosus
8
-
 leaf enzyme, second peak Ananas comosus

pH Range

pH Minimum pH Maximum Comment Organism
6 8 leaves present two peaks at pH 6.0 and pH 8.0 Ananas comosus

General Information

General Information Comment Organism
physiological function antiproliferative effect of bromelain from different tissues against B16F10 murine melanoma cells, overview Ananas comosus