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Literature summary for 3.4.22.32 extracted from

  • Ramli, A.N.M.; Manas, N.H.A.; Hamid, A.A.A.; Hamid, H.A.; Illias, R.M.
    Comparative structural analysis of fruit and stem bromelain from Ananas comosus (2018), Food Chem., 266, 183-191 .
    View publication on PubMed

Application

Application Comment Organism
medicine bromelain has gained wide acceptance and compliance as a phytotherapeutical drug. Cysteine proteases in pineapple (Ananas comosus) plants are phytotherapeutical agents that demonstrate anti-edematous, anti-inflammatory, anti-thrombotic and fibrinolytic activities Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus P14518
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Ananas comosus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyl-Phe-Val-Arg-4-nitroanilide + H2O
-
Ananas comosus benzyl-Phe-Val-Arg + 4-nitroaniline
-
?
benzyloxycarbonyl-Arg-Arg-4-nitroanilide + H2O preferred substrate of stem bromelain Ananas comosus benzyloxycarbonyl-Arg-Arg + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
? 23400-35730, stem bromelain, SDS-PAGE Ananas comosus
More comparative structural analysis of fruit and stem bromelain from Ananas comosus Ananas comosus

pI Value

Organism Comment pI Value Maximum pI Value
Ananas comosus isoelectric focusing
-
9.55

General Information

General Information Comment Organism
additional information comparative structural analysis of fruit and stem bromelain from Ananas comosus, structure homology modelling, model domain organisation, overview. The proteolytic fraction of pineapple stem is termed stem bromelain, while the one presents in the fruit is known as fruit bromelain (EC 3.4.22.33). NCBI conserved domain analysis reveals two domains of fruit and stem bromelain: cathepsin propeptide inhibitor (I29) and peptidase C1 Ananas comosus