Application | Comment | Organism |
---|---|---|
food industry | stem bromelain immobilized on chitosan beads without glutaraldehyde yields a food-safe biocatalyst for unstable real wine future application | Ananas comosus |
General Stability | Organism |
---|---|
immobilized bromelain shows a higher half-life respect to the free enzyme. The addition of free cysteine during immobilization phase, improves bromelain half-life more than 4folds | Ananas comosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2497 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, at pH 3.2 and 25°C | Ananas comosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ananas comosus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stem | - |
Ananas comosus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Bz-Phe-Val-Arg-4-nitroanilide + H2O | - |
Ananas comosus | Bz-Phe-Val-Arg + 4-nitroaniline | - |
? | |
Z-Arg-Arg-4-nitroanilide + H2O | - |
Ananas comosus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.03 | - |
Bz-Phe-Val-Arg-4-nitroanilide | free enzyme, at pH 3.2 and 25°C | Ananas comosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.2 | 7 | stem bromelain solubilized at pH 7.0 and at pH 3.2 retains, after 16 h at 256°C, 10 and 40% of the initial activity, respectively | Ananas comosus |