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Literature summary for 3.4.22.32 extracted from

  • Amid, A.; Ismail, N.; Yusof, F.; Salleh, H.
    Expression, purification, and characterization of a recombinant stem bromelain from Ananas comosus (2011), Process Biochem., 46, 2232-2239.
No PubMed abstract available
Search for more literature for 3.4.22.32

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the pENTR/TEV/D-TOPO vector, then sub-cloned into the pDEST17 expression vector. Expression in Escherichia coli BL21 Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 SDS-PAGE, recombinant protein Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gelatin + H2O
-
 Ananas comosus ?
-
 ?
Nalpha-CBZ-L-lysine 4-nitrophenyl ester + H2O
-
 Ananas comosus Nalpha-CBZ-L-lysine + 4-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
stem bromelain
-
 Ananas comosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Ananas comosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 recombinant protein stored at 4°C shows a loss in enzymatic activity. The percentage loss in recombinant bromelain enzymatic activity is 27.5% Ananas comosus
100
-
 recombinant bromelain shows no activity after 30 min of incubation at 100°C Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
-
 Ananas comosus