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Literature summary for 3.4.22.32 extracted from
- Identification and characterization of functional intermediates of stem bromelain during urea and guanidine hydrochloride unfolding (2007), J. Biochem., 141, 251-259.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Guanidine-HCl | at 2 mM | Ananas comosus |  |
| Urea | at 5 M and 8-9 M | Ananas comosus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Guanidine-HCl | complete loss of activity at 6 M guanidine-HCl | Ananas comosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.129 | - |
casein | native enzyme, at pH 7.0 | Ananas comosus | |
| 0.217 | - |
casein | native enzyme, in the presence of 5 M urea at pH 7.0 | Ananas comosus | |
| 0.221 | - |
casein | native enzyme, in the presence of 2 M guanidine-HCl at pH 7.0 | Ananas comosus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | enzyme stabilized in 60-70% ethanol is non-functional, but retains all the elements of secondary structure | Ananas comosus |
| guanidine-HCl | activity-enhanced state in the presence of 2 M guanidine-HCl | Ananas comosus |
| urea | activity-enhanced state in the presence of 5 M and 8-9 M urea | Ananas comosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ananas comosus | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Ananas comosus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Bromelain | - |
Ananas comosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.41 | - |
casein | native enzyme, at pH 7.0 | Ananas comosus | |
| 0.97 | - |
casein | native enzyme, in the presence of 5 M urea at pH 7.0 | Ananas comosus | |
| 0.99 | - |
casein | native enzyme, in the presence of 2 M guanidine-HCl at pH 7.0 | Ananas comosus | |
| 11.72 | - |
casein | native enzyme, at pH 7.0 | Ananas comosus | |
| 17.33 | - |
casein | native enzyme, in the presence of 5 M urea at pH 7.0 | Ananas comosus | |
| 18.08 | - |
casein | native enzyme, in the presence of 2 M guanidine-HCl at pH 7.0 | Ananas comosus | |
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