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Literature summary for 3.4.22.32 extracted from

  • Harrach, T.; Eckert, K.; Maurer, H.R.; Machleidt, I.; Machleidt, W.; Nuck, R.
    Isolation and characterization of two forms of an acidic bromelain stem proteinase (1998), J. Protein Chem., 17, 351-361.
    View publication on PubMed
Search for more literature for 3.4.22.32

Inhibitors

Inhibitors Comment Organism Structure
cystatin chicken, weak inhibition Ananas comosus
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane i.e. E-64, slow inactivation Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
0.0039
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
0.0074
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
0.0081
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
0.0554
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
0.0636
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23550
-
 isozyme SBA7A, mass spectroscopy Ananas comosus
23560
-
 isozyme SBA/b, mass spectroscopy Ananas comosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Ananas comosus
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
 2 isozymes SBA/a and SBA/b
-

Oxidation Stability

Oxidation Stability Organism
oxidation of the active site thiol leads to the corresponding sulfinic acid, which is catalytically inactive, irreversible oxidative degradation may lead to reduced activity of isozyme SBA/b with substrate Pyr-Phe-Lys-4-nitroanilide Ananas comosus

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein both isozymes are highly glycosylated, isozyme SBA/a is about 2fold more glycosylated than isozyme SBA/b, glycoslyation composition Ananas comosus

Purification (Commentary)

Purification (Comment) Organism
2 isozymes SBA/a and SBA/b Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem crude bromelain extract Ananas comosus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
 partially purified enzyme, 37°C, pH 6.5 Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide + H2O synthetic substrate Ananas comosus ?
-
 ?
benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide + H2O synthetic substrate Ananas comosus ?
-
 ?
benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide + H2O synthetic substrate Ananas comosus ?
-
 ?
protein + H2O
-
 Ananas comosus peptides
-
 ?
Pyr-Phe-Lys-4-nitroanilide + H2O isozyme SBA/b is about 3fold less active than isozyme SBA/a Ananas comosus Pyr-Phe-Lys + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 23550-27900, isozyme SBA/a, mass spectroscopy and SDS-PAGE, 1 * 23560-27800, isozyme SBA/b, mass spectroscopy and SDS-PAGE Ananas comosus

Synonyms

Synonyms Comment Organism
acidic bromelain stem proteinase
-
 Ananas comosus
Pineapple stem bromelain
-
 Ananas comosus
SBA
-
 Ananas comosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.024
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
0.04
-
 benzyloxycarbonyl-Arg-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
0.49
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
0.56
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
5.1
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/a, pH 6.0, 25°C Ananas comosus
6.08
-
 benzyloxycarbonyl-Phe-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus
6.4
-
 benzyloxycarbonyl-Phe-Val-Arg-NH-4-methylcoumarin 7-amide isozyme SBA/b, pH 6.0, 25°C Ananas comosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 substrate-dependent Ananas comosus

pH Range

pH Minimum pH Maximum Comment Organism
2 8
-
 Ananas comosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information k2/Ki for E-64 Ananas comosus
0.1
-
 chicken cystatin both isozymes, pH 6.0, 25°C Ananas comosus

pI Value

Organism Comment pI Value Maximum pI Value
Ananas comosus isoelectric focusing, isozyme SBA/b, and isozyme SBA/a variant 2
-
 4.8
Ananas comosus isoelectric focusing, isozyme SBA/a variant 1
-
 4.9