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Literature summary for 3.4.22.31 extracted from

  • Rowan, A.D.; Buttle, D.J.
    Pineapple cysteine endopeptidases (1994), Methods Enzymol., 244, 555-568.
    View publication on PubMed
Search for more literature for 3.4.22.31

Inhibitors

Inhibitors Comment Organism Structure
chicken cystatin strong Ananas comosus
L-3-carboxy-2,3-trans-epoxypropionylleucylamido(4-guanidino)butane i.e. E-64 Ananas comosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0131
-
 benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus

Organism

Organism UniProt Comment Textmining
Ananas comosus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glycoprotein Ananas comosus

Purification (Commentary)

Purification (Comment) Organism
-
 Ananas comosus

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Ananas comosus
-

Storage Stability

Storage Stability Organism
-20°C, stored dry, stable Ananas comosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
 Ananas comosus fragments of azocasein
-
 ?
Benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O
-
 Ananas comosus Benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
hide powder azure + H2O
-
 Ananas comosus hydrolyzed hide powder azure
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
63.8
-
 benzoyl-Phe-Val-Arg 4-methylcoumarin 7-amide
-
 Ananas comosus