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Literature summary for 3.4.22.30 extracted from

  • Noble, M.A.; Gul, S.; Verma, C.S.; Brocklehurst, K.
    Ionization characteristics and chemical influences of aspartic acid residue 158 of papain and caricain determined by structure-related kinetic and computational techniques: multiple electrostatic modulators of active-centre chemistry (2000), Biochem. J., 351, 723-733.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.30

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Carica papaya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein + H2O Carica papaya
-
 peptides
-
 ?

Organism

Organism UniProt Comment Textmining
Carica papaya
-
 purified enzyme
-

Reaction

Reaction Comment Organism Reaction ID
hydrolysis of proteins with broad specificity for peptide bonds, similar to those of papain and chymopapain mechanism, Asp158 is involved in catalysis Carica papaya
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-N-benzoyl-L-arginine 4-nitroanilide + H2O
-
 Carica papaya alpha-N-benzoyl-L-arginine + 4-nitroaniline
-
 ?
protein + H2O
-
 Carica papaya peptides
-
 ?

Synonyms

Synonyms Comment Organism
Papaya proteinase OMEGA
-
 Carica papaya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 determination of minimum energy conformation, interaction of ionized and un-ionized side chains of Asp158 and their microenvironment Carica papaya
25
-
 assay at Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pH profile Carica papaya