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Literature summary for 3.4.22.3 extracted from

  • Monter, B.; Herzog, B.; Stehle, P.; Furst, P.
    Kinetically controlled synthesis of dipeptides using ficin as biocatalyst (1991), Biotechnol. Appl. Biochem., 14, 183-181.
    View publication on PubMed

Application

Application Comment Organism
synthesis kinetically controlled formation of peptide bonds by coupling the ester substrates benzyloxycarbonyl-Ala methyl ester and benzyloxycarbonyl-Gly methyl ester with L-Ala, D-Ala, L-Gln, D-Gln and L-Cys(acetamidomethyl) respectively Ficus carica

Organism

Organism UniProt Comment Textmining
Ficus carica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Ficus carica

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
Ficus carica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Ala-methyl ester + Ala
-
Ficus carica benzyloxycarbonyl-Ala-Ala + ?
-
?
benzyloxycarbonyl-Ala-methyl ester + Ala
-
Ficus carica benzyloxycarbonyl-Ala-D-Gln + ?
-
?
benzyloxycarbonyl-Ala-methyl ester + Cys(acetamidomethyl)
-
Ficus carica benzyloxycarbonyl-Ala-Cys(acetamidomethyl) + ?
-
?
benzyloxycarbonyl-D-Ala-methyl ester + Ala
-
Ficus carica benzyloxycarbonyl-Ala-D-Ala + ?
-
?
benzyloxycarbonyl-Gln-methyl ester + Ala
-
Ficus carica benzyloxycarbonyl-Ala-Gln + ?
-
?
benzyloxycarbonyl-Gly-methyl ester + Ala
-
Ficus carica benzyloxycarbonyl-Gly-Ala + ?
-
?
benzyloxycarbonyl-Gly-methyl ester + Cys(acetamidomethyl)
-
Ficus carica benzyloxycarbonyl-Gly-Cys(acetamidomethyl) + ?
-
?
benzyloxycarbonyl-Gly-methyl ester + Gln
-
Ficus carica benzyloxycarbonyl-Gly-Gln + ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Ficus carica

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 50% of maximal activity, pH 9.0: about 55% of maximal activity Ficus carica