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Literature summary for 3.4.22.3 extracted from
- Studies on proteinases from Ficus carica var. Horaishi. V. Purification and properties of a sugar-containing proteinase (ficin S) (1974), Biochim. Biophys. Acta, 350, 38-47.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Cys | activates | Ficus carica |  |
| mercaptoethanol | activates | Ficus carica | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Ficus carica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | - |
Ficus carica | |
| HgCl2 | - |
Ficus carica | |
| I2 | - |
Ficus carica | |
| iodoacetic acid | - |
Ficus carica | |
| Na2S4O6 | - |
Ficus carica | |
| PCMB | - |
Ficus carica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
x * 26000, SDS-PAGE | Ficus carica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ficus carica | - |
var. Horaishi | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | sugar content 4.8%, sugar is tightly bound to enzyme protein | Ficus carica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ficus carica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | - |
Ficus carica | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 2.0-8.0, stable for 20 h | Ficus carica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | hydrolysis ratio is 13% at 48 h | Ficus carica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 26000, SDS-PAGE | Ficus carica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ficin S | - |
Ficus carica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Ficus carica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
30 min, stable below | Ficus carica |
| 67 | - |
30 min, complete inactivation | Ficus carica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ficus carica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | pH 6.0: about 50% of maximal activity, pH 11.0: about 35% of maximal activity, hydrolysis of casein | Ficus carica |
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Release 2023.1 (January 2023)
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