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Literature summary for 3.4.22.3 extracted from

  • Baeyens-Volant, D.; Matagne, A.; El Mahyaoui, R.; Wattiez, R.; Azarkan, M.
    A novel form of ficin from Ficus carica latex Purification and characterization (2015), Phytochemistry, 117, 154-167 .
    View publication on PubMed
Search for more literature for 3.4.22.3

Inhibitors

Inhibitors Comment Organism Structure
E-64 0.01 mM, complete inhibition Ficus carica
HgCl2 1 mM, complete inhibition Ficus carica
iodoacetamide 3 mM, complete inhibition Ficus carica
additional information ficin E is not affected by Mg2+, Ca2+ and Mn2+ at a concentration up to 10 mM. It is unaffected by synthetic (Pefabloc SC, benzamidine) and by natural proteinaceous (aprotinin) serine proteases inhibitors, by aspartic proteases inhibitors (pepstatin A) and by metallo-proteases inhibitors (EDTA, EGTA) Ficus carica
o-phenanthroline
-
 Ficus carica
TLCK 0.1 mM, complete inhibition Ficus carica
TPCK 0.1 mM, complete inhibition Ficus carica
Zn2+ 1 mM, complete inhibition Ficus carica

Metals/Ions

Metals/Ions Comment Organism Structure
additional information ficin E is not affected by Mg2+, Ca2+ and Mn2+ at a concentration up to 10 mM Ficus carica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24294
-
 mass spectrometry Ficus carica
27000
-
 SDS-PAGE Ficus carica

Organism

Organism UniProt Comment Textmining
Ficus carica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
combination of cation-exchange chromatography on SP-Sepharose Fast Flow, Thiopropyl Sepharose 4B and FPLC-gel filtration chromatography Ficus carica

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Ficus carica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O
-
 Ficus carica benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Ficus carica benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
Boc-Ala-Ala-Gly-4-nitroanilide + H2O
-
 Ficus carica Boc-Ala-Ala-Gly + 4-nitroaniline
-
 ?
Boc-Gln-Ala-Arg-7-amido-4-methylcoumarin + H2O
-
 Ficus carica Boc-Gln-Ala-Arg + 7-amino-4-methylcoumarin
-
 ?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
-
 Ficus carica Nalpha-benzoyl-DL-arginine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 27000, SDS-PAGE Ficus carica

Synonyms

Synonyms Comment Organism
ficin E
-
 Ficus carica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Ficus carica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60 30°C: about 40% of maximal activity, 60°C: about 80% of maximal activity Ficus carica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Ficus carica

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 pH 5.0: about 40% of maximal activity, pH 7.5: about 50% of maximal activity Ficus carica