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Literature summary for 3.4.22.29 extracted from

  • Liebig, H.D.; Ziegler, E.; Yan, R.; Hartmut, K.; Klump, H.; Kowalski, H.; Blaas, D.; Sommergruber, W.; Frasel, L.; Lamphear, B.; Rhoads, R.; Kuechler, E.; Skern, T.
    Purification of two picornaviral 2A proteinases: interaction with eIF-4 gamma and influence on in vitro translation (1993), Biochemistry, 32, 7581-7588.
    View publication on PubMed
Search for more literature for 3.4.22.29

Cloned(Commentary)

Cloned (Comment) Organism
coxsackievirus B4 coxsackievirus
human rhinovirus type 2 Human rhinovirus sp.

Inhibitors

Inhibitors Comment Organism Structure
antipain peptide or eIF cleavage coxsackievirus
antipain human rhinovirus; peptide or eIF cleavage Human rhinovirus sp.
chymostatin
-
 coxsackievirus
chymostatin human rhinovirus Human rhinovirus sp.
Elastinal
-
 coxsackievirus
Elastinal human rhinovirus Human rhinovirus sp.
additional information no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64) coxsackievirus
additional information no inhibition by trans-epoxysuccinyl-L-leucylamido(4-guanidino)-butane (i.e. E-64) Human rhinovirus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 Glu-Arg-Ala-Ser-Leu-Ile-Thr-Thr-Gly-Pro-Tyr-Gly-His-Gln-Ser-Gly coxsackievirus B4 coxsackievirus
0.52
-
 Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Tyr-Val-His human rhinovirus type 2 Human rhinovirus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 coxsackievirus
-
-
soluble
-
 Human rhinovirus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16300
-
 x * 16300, SDS-PAGE Human rhinovirus sp.
16700
-
 coxsackievirus B4, gel filtration coxsackievirus
17000
-
-
 coxsackievirus
17000
-
 human rhinovirus type 2, gel filtration in 0.05 M Tris, 1 mM EDTA, 5 mM DTT, 5% glycerol, with 0.5 M NaCl at pH 5.9 Human rhinovirus sp.
30000
-
 human rhinovirus type 2, gel filtration in 0.05 M Tris, 1 mM EDTA, 5 mM DTT, 5% glycerol, with 0.05 M NaCl at pH 8, behaving as a dimer Human rhinovirus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
eukaryotic translation initiation factor 4F p220 subunit + H2O coxsackievirus involved in shut-off in translation of cellular mRNAs upon viral infection, induces cleavage of eukaryotic initiation factor (eIF)4gamma component (i.e. p220) of eIF-4, formerly eIF-4F ?
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O Human rhinovirus sp. involved in shut-off in translation of cellular mRNAs upon viral infection, induces cleavage of eukaryotic initiation factor (eIF)4gamma component (i.e. p220) of eIF-4, formerly eIF-4F ?
-
 ?

Organism

Organism UniProt Comment Textmining
coxsackievirus
-
 B4
-
Human rhinovirus sp.
-
 serotypes 2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme as expressed in Escherichia coli BL21(DE3)pLysE bearing pET8c/HRV2 2A Human rhinovirus sp.
recombinant enzyme, as expressed in Escherichia coli BL21(DE3)pLysE bearing pET8c/CVB4 2A coxsackievirus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.8
-
 human rhinovirus type 2 Human rhinovirus sp.

Storage Stability

Storage Stability Organism
0°C, extremely unstable in crude extracts with a half-life of 2 h, 20% ethanol stabilizes, 4°C, 5 mg enzyme/ml, several months coxsackievirus
0°C, extremely unstable in crude extracts with a half-life of 2 h, 20% ethanol stabilizes, 4°C, 5 mg enzyme/ml, several months Human rhinovirus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
eukaryotic translation initiation factor 4F p220 subunit + H2O in vitro coxsackievirus protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O in vitro Human rhinovirus sp. protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O i.e. (eIF)-4Fgamma coxsackievirus protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O i.e. (eIF)-4Fgamma Human rhinovirus sp. protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O from HeLa cell extracts coxsackievirus protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O from HeLa cell extracts Human rhinovirus sp. protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O from rabbit coxsackievirus protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O from rabbit Human rhinovirus sp. protein CPa + protein CPb
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O involved in shut-off in translation of cellular mRNAs upon viral infection, induces cleavage of eukaryotic initiation factor (eIF)4gamma component (i.e. p220) of eIF-4, formerly eIF-4F coxsackievirus ?
-
 ?
eukaryotic translation initiation factor 4F p220 subunit + H2O involved in shut-off in translation of cellular mRNAs upon viral infection, induces cleavage of eukaryotic initiation factor (eIF)4gamma component (i.e. p220) of eIF-4, formerly eIF-4F Human rhinovirus sp. ?
-
 ?
Glu-Arg-Ala-Ser-Leu-Ile-Thr-Thr-Gly-Pro-Tyr-Gly-His-Gln-Ser-Gly + H2O
-
 coxsackievirus Glu-Arg-Ala-Ser-Leu-Ile-Thr-Thr-Gly-Pro-Tyr + Gly-His-Gln-Ser-Gly
-
 ?
Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Tyr-Val-His + H2O
-
 coxsackievirus Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala + Gly-Pro-Ser-Asp-Met-Tyr-Val-His
-
 ?
Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Tyr-Val-His + H2O
-
 Human rhinovirus sp. Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala + Gly-Pro-Ser-Asp-Met-Tyr-Val-His
-
 ?

Subunits

Subunits Comment Organism
? x * 16300, SDS-PAGE Human rhinovirus sp.
monomer 1 * 16700, coxsackievirus B4, SDS-PAGE coxsackievirus