General Stability | Organism |
---|---|
insoluble enzyme can be solubilized by 0.1% sarcosyl, solubilization enhances stability at 20°C, soluble enzyme is unstable in dilute solution, bovine serum albumin stabilizes | Enterovirus C |
stable to dialysis for more than 100 h at 4°C | Enterovirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | not (10 mM, esterase activity) | Enterovirus C | |
Cd2+ | inhibits esterase activity | Enterovirus C | |
Cl- | trace amounts eliminate activity | Enterovirus C | |
Cu2+ | weak, 1 mM, inhibits esterase activity | Enterovirus C | |
iodoacetamide | inhibits esterase activity | Enterovirus C | |
leupeptin | inhibits esterase activity | Enterovirus C | |
Mg2+ | not (10 mM, esterase activity) | Enterovirus C | |
Mn2+ | 10 mM, inhibits esterase activity; weak | Enterovirus C | |
additional information | no inhibition by pepstatin (esterase activity); no inhibition by PMSF (esterase activity) | Enterovirus C | |
NEM | inhibits esterase activity | Enterovirus C | |
Pb2+ | - |
Enterovirus C | |
tosyl-L-leucine-chloromethyl ketone | inhibits esterase activity | Enterovirus C | |
tosyl-L-phenylalanine-chloromethyl ketone | inhibits esterase activity | Enterovirus C | |
Zn2+ | 1 mM, inhibits esterase activity | Enterovirus C |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Tyr-Val-His | - |
Enterovirus C |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Enterovirus C | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
x * 17000, SDS-PAGE | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
type 2 (P712-ch-2ab) | - |
Purification (Comment) | Organism |
---|---|
HeLa Ohio cells, 4-5 h after infection, to near homogeneity | Enterovirus C |
type 2 | Enterovirus C |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | infected | Enterovirus C | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
550 | - |
- |
Enterovirus C |
Storage Stability | Organism |
---|---|
0°C, extremely unstable in crude extracts with a half-life of 2 h, 20% ethanol stabilizes, 4°C, 5 mg enzyme/ml, several months | Enterovirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Leu-Gly-Gln-Met methyl ester + H2O | esterase activity | Enterovirus C | Gly-Leu-Gly-Gln-Met + CH3OH | - |
? | |
Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala-Gly-Pro-Ser-Asp-Met-Tyr-Val-His + H2O | - |
Enterovirus C | Thr-Arg-Pro-Ile-Ile-Thr-Thr-Ala + Gly-Pro-Ser-Asp-Met-Tyr-Val-His | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17000, SDS-PAGE | Enterovirus C |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | - |
t1/2 of esterase activity: more than 100 h | Enterovirus C |
20 | - |
solubilization enhances stability at 20°C | Enterovirus C |
25 | - |
t1/2 of esterase activity: 2 h | Enterovirus C |
37 | - |
t1/2 of esterase activity: 5 min | Enterovirus C |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.6 | esterase activity | Enterovirus C |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
below, irreversible inactivation, esterase activity | Enterovirus C |